We hope in the future to include other diagnostic tests, for example, serum vitamin B1 levels, RBC transketolase
activity, homocysteine, methylmalonic acid, and blood vitamin B6 levels.
is a universal amphibolic enzyme catalyzing reactions in the Calvin cycle and the oxidative pentose pathway producing erythrose-4-phosphate, leading to phenylpropanoid metabolism.
Piacentini, "Occurrence of transketolase
abnormalities in extracts of foreskin fibroblasts from patients with Alzheimer's disease," Biochemical and Biophysical Research Communications, vol.
The corneal epithelial cells accumulate high levels of taxon-specific corneal crystallins aldehyde dehydrogenase 3A1 (Aldh3a1) and transketolase
(Tkt), which account for about 50% and 10% of water-soluble proteins, respectively [9-11], along with structural proteins such as keratin-12 .
Thiamine and transketolase
levels are reduced in diabetics as compared to healthy controls3.
Thus, if the cell requires a significant amount of reducing power (NADPH), R5P will be converted back to F6P and GAP by transketolase
Vitamin B1 status assessed by direct measurement of thiamin pyrophosphate in erythrocytes or whole blood by HPLC: comparison with erythrocyte transketolase
Thiamine Deficiency and Flux Analysis of Transketolase
The nutritional status of thiamin is assessed by the erythrocyte transketolase
A diagnosis of thiamine deficiency can be supported by decreased levels of erythrocyte transketolase
, but it's important not to wait for lab results.
This finding, combined with low serum transketolase
levels in both patients, led these researchers to conclude that this complication developed due to thiamine deficiency.
In addition, the supplement group also had improved transketolase
and aspartate transaminase status.