Sixty individuals were separately euthanized with tricaine
methane sulfonate (MS-222) and stored at -20AdegC for the entire carcass body before the start of the experiment.
Salamanders were caught by hand and anaesthetised using tricaine
methanesulphonate (MS-222), one of the anaesthetics most commonly used in the field sampling of amphibians (e.g.
The drugs used for immersion anesthesia for elasmobranchs are the following: benzocaine, etomidate, metomidate, halothane-oxygen-nitrous oxide, oxygen, quinaldine and tricaine
methane sulfonate (MS-222) (Stamper, 2004; Stamper, 2007).
Sampled fish were sacrificed with an overdose of tricaine
methanesulphonate (MS-222; 1 g [L.sup.-1]).
Euthanasia was performed in accordance with the American Veterinary Medical Association Guidelines for the Euthanasia of Animals (AVMA, 2013); primary euthanasia was exposure to buffered, pharmaceutical-grade tricaine
methanesulfonate (MS-222), followed by decapitation as a secondary method.
Several substances and combinations of substances, such as alcohol, ether, barbiturates, quinaldine, tricaine
methanesulfonate (MS 222), chlorbutanol and benzocaine have been used to induce anesthesia in fish.
At 48 hpf, embryos were screened for malformations, and the morphologically normal animals were euthanized by overdose of buffered tricaine
methanesulfonate (MS-222, 200-300 mg/L) by prolonged immersion and monitored under a dissecting microscope until their hearts stopped beating (approximately 15 min).
On November 17, 2015, after several days of recovery from the artificial spawning procedure, 55 fish were anaesthetized with tricaine
methanosufonate (MS-222) and surgically implanted with MCFT2-3L (5) radio transmitters (Lotek Wireless, Inc., Newmarket, Canada).
At the appropriate time-point following environmental or pharmacological stimulus, each fish was anesthetized in tricaine
methanesulfonate (MS 222; 300 [micro]g/mL) for 4 min and then euthanized by decapitation.
Saczk, "Determination of benzocaine and tricaine
in fish fillets using BIA with amperometric detection," Electroanalysis, vol.
Animals were anesthetized via immersion in 1% tricaine
mesylate (MS222) buffered to a pH of 7.
At each sampling event, fish were killed with an overdose of tricaine