The resin Dowex AG1X8-100 with a quaternary trimethylammonium
exchange group and chloride as counter ion was selected for extraction and concentration of GLYP and AMPA and, the pH should be optimized to permits to maximum analytes retention as AMPA and GLYP may have positive and negative charge depending on pH.
Critical micelle concentrations of binary surfactant mixtures (CTAB = cetyl sodium dodecyl sulphate and trimethylammonium
bromide, NaOct = sodium octanoyate SDS Mole SDS/ SDS/ CTAB/ NaOct/ NaOct/ SDS/ fraction CTAB Tween-20 Tween-20 CTAB Tween-20 NaOCt 0 0.
The method is comprised of attaching a reactive group X--Y to a surface of a pigment; and subsequently displacing Y with an organic substrate N--S--ZM to form a surface-modified pigment having attached X--N--S--ZM; wherein X is a sulfonyl, phosphoryl, or 1,3,5-triazine group; Y is fluorine, chlorine, bromine or iodine; N is an amine, an imine, a pyridine, or a thiol group; S is substituted or unsubstituted alkyls, aryls, or polymer chains having a molecular weight range from approximately 300-8000; Z is a carboxyl, sulfonyl, phenolic, phosphoryl, ammonium, trimethylammonium
, or tri-butylammonium group; and M is a halide, a negatively charged ion, a proton n salt form, or a cation in salt form.
For example, acrylic acid, sodium acrylamidopropane sulfonate, and acryloylethoxyethyl trimethylammonium
chloride were polymerized in the presence of soyamidopropyl dimethylamine, in aqueous solution, to synthesize the desired complex.
Genomic DNA was extracted manually via precipitation with trimethylammonium
bromide salts from leukocytes contained in 450 ul of venous blood using ethylenediaminetetraacetic acid (EDTA) as an anticoagulant.
Reagents (analytical grade): 5,5'-Dithiobis(2-nitrobenzoic acid (DTNB), (2-Mercaptoethyl) trimethylammonium
iodide (thiocholine iodide), 1-chloro-2,4-dinitroben zene (CDNB), reduced glutathione (GSH), Tri chloroacetic acid (TCA), Thiobarbituric acid (TBA), Tris-HCl, Diethylene triamine pentaacetic acid (DTPA), buthylated hydroxytoluene (BHT) dipotassium phosphate and monopotassium phosphate, Bradford reagent and the Bovine g-globulin.
16 ppm corresponding to the trimethylammonium
group in addition to other functional groups (Fig.
Ligands with poly-functional group, such as ethylenediaminetetraacetic acid disodium (EDTA) and Cetyl trimethylammonium
bromide (CTAB), etc.
El protocolo de extraccion consistio en una modificacion del metodo cetil- trimethylammonium
bromide (CTAB) de Doyle & Doyle (1990).
5% hexadecyl trimethylammonium
bromide (HTBA) using a Polytron (Ultra-turrax T-25) homogenizer.