Proteomic comparison between Japanese black and holstein cattle by two-dimensional gel electrophoresis
and identification of proteins.
The patterns of synthesis of individual proteins were compared, using two-dimensional gel electrophoresis
[ILLUSTRATION FOR FIGURES 7, 8 OMITTED], for the trochophore stage (1-day-old) and a veliger stage (3-day-old).
Taken together, the results of the two-dimensional gel electrophoresis
, Western blotting, and immunodepletion experiments described above demonstrate that the 11.
Therefore the technique of electrophoresis, particularly two-dimensional gel electrophoresis
, is also very useful for the diagnosis of CMP in our Pakistani population where the work on the diagnosis of cardiovascular disease is merely conventional and highly underdeveloped.
The first major breakthrough, which was a great leap forward in separation, took place in 1975 with the introduction of two-dimensional gel electrophoresis
Eight human pituitary control tissues were used to extract proteins, and each control tissue was analyzed (three to five times) by two-dimensional gel electrophoresis
For example, two-dimensional gel electrophoresis
and MS/MS are being used for global protein separation and protein identification.
The ICPL Triplex technology was designed to provide reliable quantitation due to data redundancy and compatibility with any combination of protein fractionation technologies, including one- or two-dimensional gel electrophoresis
or protein LC.
The aim of this study was to use a serologic proteomic analysis, including one- and two-dimensional gel electrophoresis
, immunoblot analysis, and matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) for the reliable characterization and identification of putative targets of anti-SLA antibodies.