The duodenum was excised and placed in Tyrode's solution
contained in organ bath of 50 ml capacity and bubbled with 100% O217 and maintained at a temperature of 37 +- 2oC18.
The zona pellucida of unhatched blastocysts was removed with acidified Tyrode's solution
before mRNA extraction.
The ileum was cut into 2 inches pieces6 and transferred to an organ bath of 50ml capacity containing tyrode's solution
(in mM: NaCl, 136.
2+] containing tyrode's solution
consisting of (in mM) NaCl 137, KCl 5.
To check whether an active acid-extruding mechanism exists in the HUASMCs, we firstly performed the experiments in HEPES-buffered Tyrode's solution
, that is, nominally free of CO[sub]2 /HCO[sub]3 [sup]− .
Briefly, the hearts were perfused with a Tyrode's solution
The supernatant was removed and the sperm pellet resuspended in fresh Tyrode's solution
devoid of Ca[Cl.
2+] currents, the bath Tyrode's solution
was contained in mM: 136Tris-HCl, 5.
Mesenteries were removed from jejunum and immersed in Tyrode's solution
The aorta was cannulated and connected to Langendorff perfusion system filled with Tyrode's solution
saturated with 95% [O.
Moreover, in the majority of studies which have compared the effect of acidified Tyrode's solution
and laser zona drillings on subsequent embryonic development, there has been no significant difference between the two methods (13 - 15).
Three controls were defined: a) Tyrode's solution
b) brain-heart broth infusion (CCC) and c) supernatant not toxigenic of E.