Tyrode's Solution

Tyrode’s Solution

 

a balanced aqueous solution of salts and glucose whose osmotic pressure and ion concentration are similar to those of blood plasma. The solution, which belongs to the category of physiological solutions, was first developed by the American pharmacologist M. Tyrode in 1910.

References in periodicals archive ?
Tyrode's solution containing 3.5 mg/mL BSA was used to prepare the final suspension of washed human platelets.
After the electrophysiological study (EPS), the hearts were perfused on a Langendorff apparatus at 37[degrees]C by pumping with Tyrode's solution (calcium, 1.8 mmol/L).
Rabbits' jejunal preparations were mounted in organ bath containing 10 ml Tyrode's solution, constantly aerated with carbogen gas.
Cells were allowed to settle in Tyrode's solution. The solution was subsequently removed and replaced with 10 mL Tris (20 mmol, pH 7.5), then centrifuged at 94g for 3 min.
The test solution for AP recording was Tyrode's solution with 1.8 mM [Ca.sup.2+].
EOPh was dissolved in Tyrode's solution for the in vitro protocols using cremophor (0.1% v/v) as the eluent.
The dye solution was dropped into the Tyrode's solution to be the desired concentration just before the measurement.
Tyrode's solution was used for the study as a nutrient solution, the composition of which was NaCl: 137 mM (08.00 g), KCl: 2.7 mM (0.20 g), CaCl2:1.8 mM (0.20 g), MgCl2: 1.05 mM (0.10 g), NaHCO3: 12.0 mM (1.00 g), NaH2PO4: 0.42 mM (0.05 g), Glucose: 5.6 mM (1.00 g) dissolved in 1 L of distilled water.
The ileum was cut into 2 inches pieces6 and transferred to an organ bath of 50ml capacity containing tyrode's solution (in mM: NaCl, 136.8mM; KCl, 2.7mM; MgCl2, 0.5mM; CaCl2, 1.3mM; NaH2PO4, 0.14mM; NaHCO3, 12.0mM, Dextrose, 5.5mM) and aerated continuously with 95% oxygen and 5% carbondioxide.5 One end of the ileal strip was attached to the bottom of the oxygen tube in tissue bath and the other end was connected to a research grade force Displacement transducer.2 After equilibration the isotonic ileal smooth muscle activity was recorded through the Displacement Transducer on Power lab.7
The bath perfusate for sarc[K.sub.ATP] channel and AP recording was the [Ca.sup.2+] containing tyrode's solution consisting of (in mM) NaCl 137, KCl 5.4, Mg[Cl.sub.2] 1.2, Ca[Cl.sub.2] 1, Na[H.sub.2]P[O.sub.4] 1.2, HEPES 20, and glucose 10, with the pH controlled to 7.4 with NaOH.
To check whether an active acid-extruding mechanism exists in the HUASMCs, we firstly performed the experiments in HEPES-buffered Tyrode's solution, that is, nominally free of CO[sub]2 /HCO[sub]3 [sup]− .
Briefly, the hearts were perfused with a Tyrode's solution containing 0.7 mg/mL type II collagenase (Worthington Biochem).