Food and Drug Administration (FDA)-approved uricase
therapy, in adult patients with chronic refractory gout.
In a separately conducted and designed study of the only US Food and Drug Administration (FDA)-approved uricase
therapy, 44% of evaluable patients had serum uric acid control below 6 mg/dl at week 16.
Lactobacillus plantarum Mar8 is a lactic acid bacterial isolate producing uricase
that has good catalytic activity on uric acid (Iswantini et al., 2014).
Garcia-Arroyo et al., "Synergistic effect of uricase
blockade plus physiological amounts of fructose-glucose on glomerular hypertension and oxidative stress in rats," American Journal of Physiology.
Improved production of Pseudomonas aeruginosa uricase
by optimization of process parameters through statistical experimental designs.
Serum creatinine was determined using the principle of Jaffe reaction as described by Bonsnes and Tausslay , while serum urea was determined by the kit (Quinica Clinica Spam), the Uricase
method as described by Wootton .
A recombinant uricase
or xanthine oxidase inhibitor is preferred to maintain the serum urate level below 300 [micro]mol/L Emergency management is required for patients with confirmed acute uric acid nephropathy.
Serum uric acid levels were measured by standardized enzymatic PAP method with uricase
and peroxidase, in cobas C311 Autoanalyser with intra- and interassay % CV less than 2.44% and according to the procedure recommended by the manufacturer.
The deficiency in uricase
enzyme increases serum levels of uric acid that forms monosodium urate (MSU) crystals.
A rat model receiving oxonic acid, an inhibitor of uricase
, has been widely used to study the pathophysiological roles of hyperuricemia [14-17].
Unlike humans, the animals have the enzyme uricase
, which catalyzes the oxidation of uric acid .
Glew, "Enzymatic degradation of uric acid by uricase
loaded human erythrocytes," Journal of Clinical Investigation, vol.