Vacuum Infiltration

The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Vacuum Infiltration

 

a method of studying the action of enzymes in a living plant.

Vacuum infiltration involves injecting solutions of different substances into the leaves. The part of the plant under study is immersed in a solution and the air above it is completely exhausted. As a result, air escapes from the spaces between the cells; after this the solution enters the interstices of the cells under the pressure of the newly released air. This method was used to determine the rate and direction of enzymatic reactions in living tissues. The correlation between the synthesis and breakdown of substances in cells was shown to be a distinguishing characteristic of the species and variety of plant.

REFERENCES

Kursanov, A. L. “Primenenie metoda vakuum-infil’tratsii dlia kolichestvennogo opredeleniia sinteziruiushchego i gidroli-ziruiushchego deistviia invertazy v zhivykh rastitel’nykh tkaniakh.” Biokhimiia, 1936, vol. 1, issue 3.
Kursanov, A. L. Obratimoe deistvie fermentov v zhivoi rastitel’noikletke. Moscow, 1940.
The Great Soviet Encyclopedia, 3rd Edition (1970-1979). © 2010 The Gale Group, Inc. All rights reserved.
References in periodicals archive ?
The Novel Use of a Combination of Sonication and Vacuum Infiltration in Agrobacterium-mediated Transformation of Kidney Bean (Phaseolus vulgaris L.) with lea Gene.
4 5 2 Vacuum infiltration, 1 h, 1.25 plg Hg 4 3 Immersion and agitation for 15 min 3 4 Vacuum infiltration, 1 h, 1.25 plg Hg 4 5 Vacuum infiltration, 1 h, 1.25 plg Hg 4 6 Vacuum infiltration, 1 h, 1.25 plg Hg 4 7 Immersion and agitation for 15 min 3 8 Vacuum infiltration, 1 h, 1.25 plg Hg 4 TABLE 2 Recidivism of LBA4404-ElectroMAX[R] A.
For instance, in 2016, Sakura Finetek introduced "Tissue-Tek VIP 6 AI Vacuum Infiltration Processor" to prepare pathological tissue specimens.
Results indicated that out of four different treatments inoculation of calli with bacterial culture of OD6000.4 through vacuum infiltration at -50 kPa produced maximum number of transgenic plants.
Seven momarsa for the supply of (a) a cellular flow instrument required for the tissues tans plant unit, (b) a vacuum infiltration processor for the Surgical Pathology Div., (c) a chemical analyzing instrument for the Chemistry Laboratory, (d) medical instruments for the Surgical Pathology Div., (e) an orthopedic saw for the same division, (f) medical instruments for the Cancer Biology Division & (g) a research microscope for the Tissue Transplant Division.
Fully developed plugs are then transplanted to grow trays that are fitted with a vacuum infiltration lid.
Xylem sap was extracted by vacuum infiltration as described earlier [7, 28].
Protocol for vacuum infiltration transformation of Arabidopsis thaliana.
(1993)[5] conducted a breakthrough study to transform uprooted flowering plants of Arabidopsis by vacuum infiltration in agro-suspension.
They are also working on new techniques for inserting fungicide into the vines using a process known as "vacuum infiltration," which would allow them to treat grape cuttings before they are sold.
They eliminate pesticides by combining vacuum infiltration with soap solutions to kill white flies and aphids, two of the most common greenhouse pests.