In patients who are severely immunocompromised, viral inclusion bodies may be extremely abundant and demonstrate atypical features (Figure 1, c through f).
Although characteristic viral inclusion bodies in GI biopsies often may be focal and require several histologic levels for identification, their detection in significantly immunocompromised HIV patients is usually not difficult.
Comparison of the 3 Most-Common Viral Inclusion Bodies Seen in Human Immunodeficiency Virus-Associated Gastrointestinal Opportunistic Infections Cytomegalovirus Herpes Simplex Virus Cell type Endothelium, stromal, Epithelium (squamous, epithelium (glandular), less often glandular) macrophage Nucleus Nucleomegaly, eosinophilic Syncytial change, inclusion with surrounding homogenous eosinophilic halo/"owl-eye" "ground-glass" or Cowdry type-A inclusion Cytoplasm Cytomegaly, granular No inclusion basophilic inclusion bodies Adenovirus Cell type Epithelium (surface columnar, goblet cell) Nucleus Nucleomegaly, basophilic "smudged" inclusion Cytoplasm No inclusion
Immunoelectron microscopy with colloidal gold-labeled antibodies against NS3 protein and the viral nucleocapsid protein revealed in situ accumulation of NS3 protein in the cytoplasm but not in the viral inclusion bodies, vacuoles or chloroplasts of RHBV-infected plants, following the same pattern of distribution as the RHBV nucleocapsid protein.
4b and 4c), especially in collapsing cells that has already accumulated large masses of viral inclusion bodies.