While for Voges-Proskauer test
, cultured MR-VP broth was supplemented with 0.6 ml of Barritt's reagent A and 0.2 ml of Barritt's reagent B.
Biochemical analyses including pH, temperature, lactose fermentation test (LAC), indole test (IND), methyl red test (MR), Voges-Proskauer test
(VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An) were measured.
pneumoniae identified were as follows: positive citrate utilization test, negative methyl red test, negative indole test, positive urease test, positive Voges-Proskauer test
, sucrose, acid and abundant gas production from glucose, lactose, mannitol sugar fermentation tests, and maltose.
 found that hydroxyproline amidase, maltosidase, tripeptidase, proline deaminase, catalase reaction, Voges-Proskauer test
, and fermentation of glycerol, melibiose, and myoinositol were suitable parameters to separate Y regensburgei from H.
All the isolates were subjected to conventional biochemical methods (Like Indole production test, Methyl red test, Voges-Proskauer test
, Citrate utilisation test, Urease test, Triple Sugar Iron test, Sugar fermentation tests, Coagulase test, Oxidase test, and Catalase test) in accordance to the standard microbiological techniques.
lapagei can be distinguished from other Cedecea strains by its ability to grow in media lacking thiamine and because it is quickly detected in the Voges-Proskauer test
following the O' Meara method.
In addition, the pyrrolidonylarylaminase and leucine aminopeptidase tests are positive, the Voges-Proskauer test
is negative, and the organism may have variable susceptibility to bacitracin.
IMVIC is an acronym that stands for four different tests; Indole test, Methyl red test, Voges-Proskauer test
, Citrate utilization test to obtain the results of these four tests, three test tubes are inoculated: tryptone broth (indole test), methyl red/Voges-Proskauer broth (MR-VP broth), and citrate.
Test Result Comment Catalase Negative Excludes Nocardia, Nocardiopsis, Streptomyces, Mycobacterium fortuitum, Bifidobacterium dentium, Actinomyces neuii, Actinomyces viscosus Nitrate reduction Positive Excludes Actinomyces turicensis, Actinomyces radingae, Actinomyces europaeus Colony pigment/ color Grayish-white Excludes Actinomyces Non-pigmented odontolyticus Colony texture Rough Excludes Actinomyces naeslundii (geno.1), Actinomyces gerencseriae Urea hydrolysis test Negative Excludes Actinomyces naeslundii, Actinomyces viscosus Voges-Proskauer test
Negative Fermentation of Positive glucose, maltose, sucrose Fermentation of Positive Suggest Actinomyces israelii arabinose, sorbitol
To confirm additional changes in biotype attributes in the variant Kolkata isolates during 2003-2014, we used the Voges-Proskauer test
to determine production of acetylmethyl carbinol and found that the tested strains produced acetoin and were positive for chicken erythrocytes agglutination.
For biochemical characterization the isolate was tested for catalase activity, motility, oxidase acivity, nitrate reduction and Voges-Proskauer test
. Some specific tests were performed for further characterization of isolates such as sporulation test, acid formation test, utilization of different sugars, hydrolysis of casein, hydrolysis of starch, growth at 20C, 30C, 37C, 45C, 50C, growth in medium containing 2%, 5%, 7% and 10% NaCl and growth at different pH.
were identified by Gram stain, motility test, methyl red test, Voges-Proskauer test
and sugar fermentation tests.