IMVIC is an acronym that stands for four different tests; Indole test, Methyl red test, Voges-Proskauer test
, Citrate utilization test to obtain the results of these four tests, three test tubes are inoculated: tryptone broth (indole test), methyl red/Voges-Proskauer broth (MR-VP broth), and citrate.
Biochemical analyses including pH, temperature, lactose fermentation test (LAC), indole test (IND), methyl red test (MR), Voges-Proskauer test
(VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An) were measured.
1), Actinomyces gerencseriae Urea hydrolysis test Negative Excludes Actinomyces naeslundii, Actinomyces viscosus Voges-Proskauer test
Negative Fermentation of Positive glucose, maltose, sucrose Fermentation of Positive Suggest Actinomyces israelii arabinose, sorbitol
lapagei can be distinguished from other Cedecea strains by its ability to grow in media lacking thiamine and because it is quickly detected in the Voges-Proskauer test
following the O' Meara method.
In addition, the pyrrolidonylarylaminase and leucine aminopeptidase tests are positive, the Voges-Proskauer test
is negative, and the organism may have variable susceptibility to bacitracin.
To confirm additional changes in biotype attributes in the variant Kolkata isolates during 2003-2014, we used the Voges-Proskauer test
to determine production of acetylmethyl carbinol and found that the tested strains produced acetoin and were positive for chicken erythrocytes agglutination.
For biochemical characterization the isolate was tested for catalase activity, motility, oxidase acivity, nitrate reduction and Voges-Proskauer test
were identified by Gram stain, motility test, methyl red test, Voges-Proskauer test
and sugar fermentation tests.
The isolate was found to be positive for enteric bacteria in the Voges-Proskauer test
and resistant to polymyxin B (50 units).
Positive results for catalase and methyl red tests and negative results for oxidase, indole utilization tests and Voges-Proskauer tests
The isolates were positive for chicken cell agglutination and Voges-Proskauer tests
and were polymyxin B resistant, characteristics of El Tor biotype.
In addition, the 37 clinical isolates were identified as El Tor 01 on the basis of Voges-Proskauer tests
and were positive for [hlyA.