Western blotting

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Western blotting

[′wes·tərn ‚bläd·iŋ]
(cell and molecular biology)
A protein detection technique in which proteins are separated by one- or two-dimensional gel electrophoresis, transferred (blotted) to a nitrocellulose sheet, and then treated with radioactive antibodies (or antibodies coupled to a fluorescent dye or an easily detectable enzyme) that are specific to the protein of interest.
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References in periodicals archive ?
It is a device for fully automated semi-quantitative detection of proteins, particularly in tissue samples, on a Western blot.
Expression of RhoA in the cytoplasm and membrane as well as ROCK II was also determined by Western blot analysis.
Two important steps for producing reliable western blot data are normalization and signal detection.
This unique attribute is the primary reason for replacing the Western blot with the Multispot.
All samples identified as positive (n = 93) by ECLIA were confirmed as positive by Western blot.
With regard to the negative result for the Western blot, part of the original sentence was accidentally changed in the published letter (2); the correct information is "confirmatory Western blot test results were negative on days 5 and 15, and showed the p24 and p41 bands on day 23.
Multiplex Detection and Quantitation of Proteins on Western Blots Using Fluorescent Probes," BioTechniques 29, 636-642 (2000).
Three options for supplemental testing of specimens remain: the Genetic Systems Western blot kit (Biorad Laboratories Inc.
On April 17, 2002, Calypte Biomedical Corporation (Alameda, California) announced the company might stop manufacturing the Cambridge Biotech HIV-1 Western blot kit.
At that time, the HIV ELISA and Western Blot were positive.
The most often used confirmatory test is the Western blot.
The Western blot confirmatory test -- the only FDA-approved test for confirming ELISA results -- was licensed for commercial use in 1987.