B, Expression levels of migration-associated proteins were assessed by western blot analysis
. C, Cell invasion was detected with the transwell invasion assay.
For Western blot analysis
, the adopted antibodies included ENaC-a antibody (70R-13299, Fitzgerald, USA); serum- and glucocorticoid-inducible kinase 1 (SGK1) antibody (ab59337, Abcam, UK), SGK1-phosphor-S422 antibody (ab55281, Abcam), [sz]-actin antibody AC-15 (ab6276 Abcam), goat anti-rabbit IgG H&L HRP-conjugated antibody (ab6721, Abcam), and goat anti-mouse IgG-Fc fragment HRP-conjugated antibody (Bethyl, USA).
Western blot analysis
for PrP with odd and even number lanes showing reaction mixtures before and after protein misfolding cyclic amplification.
EI24 mRNA was downregulated in the poorly differentiated PDAC cell lines (AsPC-1 and PANC-1) compared with moderately to well-differentiated cells CFPAC-1, CaPan-1, CaPan-2, and SW1990, and Western blot analysis
was consistent with these results (Figure 2(a)).
Western blot analysis
by using tumor lysates showed that silenced PPAR[alpha] reduced LC3-II levels and increased Bcl2 protein levels (Figure 4(c)).
The western blot analysis
showed an increased expression of Beclin-1 and LC3-II and a decrease expression of p62 in the NP cells treated with PPAR[gamma] agonist when compared with the 0.2 M high glucose condition (Figure 4).
(b) The total protein was extracted from the lung tissue of mice for Western blot analysis
. (c, d, e) The expression of COL I, COL III, and [alpha]-SMA in the lung tissue of mice was determined by Western blot analysis
, with GAPDH as an internal reference (* P < 0.05 versus control group, (#) P < 0.05 versus Ova-treated group).
Caption: Figure 3: Comparison of OxiDJ-1 levels from patient urine samples using Western blot analysis
. (a) Urine samples of non-PD controls (34, 35, 36, 39, 41, 43, 44, 46, 50, 55, 60, 101, 105, 108, 109, 112, 113, 115, 116, 117, 120, and 121) or PD patient (1, 2, 3, 4, 5, 9, 10, 12, 13, 15, 17, 20, 21, 23, 24, 25, 28, 29, 30, 71, 72, 74, 75, 76, 77, 78, 79, 80, 81, 84, 86, 88, and 89) were subjected to Western blot.
ASCs were further characterized for the expression of mesenchymal and epithelial markers (vimentin and cytokeratin 14, resp.) by both immunofluorescence and Western blot analysis
, to confirm the absence of epithelial contaminants.
(a) Representative and statistical results of Western blot analysis
showed the membrane translocation of cPKC[beta]II in the cortex, hippocampus, and striatum at 2 weeks after STZ injection.
Caption: Figure 2: One-step quantitative real-time polymerase chain reaction (qPCR) test and Western blot analysis
were performed to detect the mRNA and protein expression of Rab27b in lung adenocarcinoma (LUAD) cell lines and tissue samples.
(Guangzhou, China) for mass spectrometry (MS) detection and bioinformatic analysis, and the other was for Western blot analysis