Western blotting


Also found in: Dictionary, Medical, Acronyms.

Western blotting

[′wes·tərn ‚bläd·iŋ]
(cell and molecular biology)
A protein detection technique in which proteins are separated by one- or two-dimensional gel electrophoresis, transferred (blotted) to a nitrocellulose sheet, and then treated with radioactive antibodies (or antibodies coupled to a fluorescent dye or an easily detectable enzyme) that are specific to the protein of interest.
Mentioned in ?
References in periodicals archive ?
Brazil, Mexico and countries in rest of Latin America together form the market for western blotting in the Latin American region.
Assessing intra-assay variability of phosphorylation p70S6 kinase by Western blotting
Although a more elegant solution, multiplex fluorescent western blotting requires optimization of blocking reagents, antibody concentrations and incubation times, while users need to be mindful of challenges like antibody cross-reactivity.
To understand whether the effects of SHS on rat hearts are mediated through activating apoptotic pathways, including mitochondria-dependent and Fas death-receptor-dependent signalings, or through suppressing survival pathways, we examined the cardiac levels of signaling proteins and gene expression in these pathways by performing reverse-transcriptase polymerase chain reaction (RT-PCR), Western blotting, or dot blotting.
The "North America Western Blotting Market 2019-2027" report has been added to ResearchAndMarkets.com's offering.
Extensively used techniques in immunology and biotechnology like ELISA and Western Blotting entirely rely on detection of proteins and antigens by antibodies.
Western blotting, also known as protein blotting or immunoblotting, is a technique under molecular biology that is used to detect and analyze proteins.
Northern and Western Blotting. Northern and Western blots were performed as we have previously described [5,10].
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
Fluorescent Western blotting can detect multiple proteins in a single assay.