Western blotting

(redirected from Western transfer)
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Western blotting

[′wes·tərn ‚bläd·iŋ]
(cell and molecular biology)
A protein detection technique in which proteins are separated by one- or two-dimensional gel electrophoresis, transferred (blotted) to a nitrocellulose sheet, and then treated with radioactive antibodies (or antibodies coupled to a fluorescent dye or an easily detectable enzyme) that are specific to the protein of interest.
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The presence of P2X7 receptors on [GH.sub.4][C.sub.1] cells was additionally supported by the immunostaining of Western transfers of [GH.sub.4][C.sub.1] cell membranes using an antibody specific to the unique carboxy terminal domain of the P2X7 receptor.

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