To examine whether the spike mutations affect sensitivity to the neutralizing activity of antibodies against wild-type spike, we investigated whether the antibodies generated in mice immunized with wild-type spike antigen are able to neutralize the spike mutant viruses as efficiently as wild-type virus
. We performed a 50% pseudoparticle neutralization test assay on wild-type and mutant spike-pseudotyped lentiviruses and then compared their neutralizing efficacy.
Thus, in this study we generated and evaluated in a mouse model a pair of H1N1 LAIVs containing NP either from MDV (6: 2 LAIV) or from wild-type virus
(5: 3 LAIV).
The RT PR sequences obtained from the patient are compared to the RTPR sequences of the wild-type virus
and examined for mutations at resistance sites.
Herpes zoster associated with the Oka vaccine strain has been documented in 23 vaccinees; another 15 turned out to have wild-type virus
Apparently he too had reverted to the wild-type virus
: His T cells are now back up over 200, and his viral load is a manageable 400.
Infectious virus may be identified in the feces as early as 4 days after the intravenous injection of infectious material in susceptible primates (7 days in animals fed wild-type virus
orally), and continues to increase in magnitude until just before the onset of chemical evidence of liver disease.
UDL-01/08 was compared to UDL-01/08 HA backbone variants with A180T and A180V substitutions, and wild-type virus
SKP-827/16 was compared with SKP-827/16 HA backbone variants with T180A and T180V substitutions.
We found that when the new vaccine, containing gene sequences similar to the wild-type virus
, was used in vaccination studies, the vaccinated birds were protected from disease and shed less of the wild-type virus
after challenge," says Yu.
The wild-type virus
is found in the natural infection, in contrast to the virus strain found in vaccine.
In addition, the vaccine strain may reactivate, causing a zoster-like illness similar to that caused by reactivation of the wild-type virus
Virological failures were 11 (7%) versus 5 (3%) with evidence of one case of wild-type virus
in both arms and one subject with an emergent PI resistance at failure (10I/V, 48V, 54V, 82A, 84V) in the SQV/r arm.
The risk could take the form of either wild-type virus
escaping from a facility or vaccine-derived polioviruses.