Tuberculous mycobacteria bacilli fluorescence and compare with Ziehl-Neelsen stain
in fine-needle aspiration cytology of tubercular lymphnode.
Additionally, the lesions in the mediastinal lymph nodes and the pulmonary nodules are grossly compatible with tuberculosis which is an important differential diagnosis that was ruled out through histopathology as well as negative results in the Ziehl-Neelsen stain
Comparison of fluorescence microscopy with Ziehl-Neelsen stain
for demonstration of acid-fast bacilli in smear preparations and tissue sections.
However, in the microbiology laboratory a Ziehl-Neelsen stain
with a small sample obtained from a cutaneous biopsy was carried out, but no fast-acid bacilli (FAB) were observed.
Laboratory examination of the pus showed Gram-positive Bacilli, and a modified Ziehl-Neelsen stain
showed partially acid-fast Bacilli without true branching.
A Ziehl-Neelsen stain
for acidoresistant bacteria was negative in three sputum samples and negative in one bronchial lavage sample.
Positive fluorochrome stains were confirmed using a Ziehl-Neelsen stain
. The specimens were inoculated into Mycobacteria Growth Indicator Tubes (Becton Dickinson Diagnostics, Sparks, Maryland) containing a modified Middlebrook 7H9 broth base and onto Middlebrook 7H11 agar (both from Becton Dickinson Diagnostics, Sparks, Maryland) with and without antibiotics.
Representative sections of liver, spleen, heart, lungs, air sacs, kidneys, intestines, gonads, adrenal glands, thyroid gland, trachea, esophagus, ventricle, proventriculus, crop, skeletal muscle, and bone marrow were fixed in 10% buffered formalin and stained with hematoxylin and eosin and Ziehl-Neelsen stain
. Birds were considered cured if no macroscopic or microscopic lesions consistent with active mycobacteriosis were identified in any organ; the liver, spleen, and bone marrow were culture and PCR negative; and acid-fast organisms were not seen in Ziehl-Neelsen-stained sections in any organ.
(i) the histology of a nodule, an oedema, or an infiltrated plate with Ziehl-Neelsen stain
However, Ziehl-Neelsen stain
has a relatively low sensitivity for detecting Mtb with sensitivity range of zero to 44% for this infection .
The presence of acid-fast bacilli was confirmed with a Ziehl-Neelsen stain
(5) The organisms may be acid-fast with the Ziehl-Neelsen stain
, however, this too depends on its growth media and the age of the culture.