The fluid protein was 4.8 g%, sugar 92 mg% and fluid was negative for acid-fast bacilli on Ziehl-Neelsen staining
. Adenosine deaminase activity level in the fluid was 14.0 U/L.
The Auramine-O staining technique is more sensitive than conventional Ziehl-Neelsen staining
method as even low bacillary load can be diagnosed easily under fluorescent microscope and it has been superior to the ZN staining.
Diagnosis requires observation of tubercle bacilli in the Ziehl-Neelsen staining
of the Cerebro Spinal Fluid (CSF) but it lacks sensitivity.
However, subsequent tissue biopsies revealed Langhans giant cells and epithelioid cell granulomata, which were consistent with the histopathologic evidence of tuberculosis, although acid fast bacilli were absent when Ziehl-Neelsen staining
of the tissues was performed.
These staining techniques are beneficial as they are easy to do and have high sensitivity and specificity.6 For rapid identification of fungal pathogens, conventional techniques include 10% KOH and lacto phenol cotton blue wet mounts.8 Gram and Giemsa staining techniques are used for identification of bacteria6 and Modified Ziehl-Neelsen staining
for Nocardia identification.9 These staining techniques provide a preliminary diagnosis, when culture results are pending.10
Validity of GeneXpert and rifampicin resistance were determined and compared with Ziehl-Neelsen staining
using Lowenstein-Jensen culture as the gold standard.
For histopathological analysis, histological sections of tissue biopsies with a thickness of 5 [micro]m embedded in paraffin were stained by hematoxylin-eosin, Ziehl-Neelsen staining
, and subsequent tissue immunostaining with specific monoclonal antibodies.
Other tests which can be useful in diagnosing peritoneal TB include culture of the fluid, Ziehl-Neelsen staining
, and PCR assay for mycobacteria.
Direct microscopy of the tissue samples after Ziehl-Neelsen staining
was negative for acid fast bacilli (AFB).
Material and Methods: A cross sectional study in which four hundred and eighty sputum samples from MDR-TB patients' attendants were processed for the detection of M.TB through Ziehl-Neelsen staining
, Lowenstein-Jensen, BACTEC MGIT-960 culture and line probe assay.
indicated they were acid-fast bacilli.