All the patients were asked for permission to include their 3PN zygotes
in the study.
Data on proportion of morula or blastocyst stages are presented as ratio of the numbers of morula or blastocysts to the number of cleaved zygotes
were fixed with 3% paraformaldehyde and 0.01% Triton X in 100 mM PIPES buffer (pH 6.8) containing 5 mM EDTA and 1 mM Mg[Cl.sub.2] (pH 6.8) at 4[degrees]C (slightly modified from Pielak et al.
Takemoto, "Electroporation enables the efficient mRNA delivery into the mouse zygotes
and facilitates CRISPR/Cas9-based genome editing," Scientific Reports, vol.
Instead, the zygote
utilized the maternal copy of the gene with the normal sequence as a template for repairing the DNA cut in the paternal copy produced by CRISPR.
To determine whether the SAC is engaged in response to oxidative stress damage in zygotes
, we examined the localization of mitotic arrest deficient 2-like 1 (MAD2L1; also known as MAD2) and the protein kinase TTK by immunofluorescence staining.
Sylwester wants names on documents in support of zygotes
? Instead, I suggest that he show responsibility by signing his name in support of legislation that supports reproductive health and safety.
Therefore, even if the current state of technology does not permit the therapeutic correction of genetic diseases by means of editing via zygote
injection, the two approaches suggested by Porteus and Dann could be attempted, even for genetically dominant diseases.
Section 57(3) provides that '[n]o person may import or export human zygotes
or embryos without the prior written approval of the Minister.
The incubation period, the fixation and counting of zygotes
followed the same procedure previously described.
The culture of ovine zygotes
in IMO with two highly efficient ruminant embryo culture media (SOFaaciBSA and SOFaaBSA), viability of explanted oviduct, cultured zygotes
development and quality of resulted embryos have not been studied to date.