Caption: FIGURE 3: Correlations between the intensities of each
mass-to-charge ratio (m/z) and each urinary protein/urinary creatinine (UP/UCr) ratio.
This approach is highly selective and only molecular species with the desired combination of the precursor and product ion
mass-to-charge ratio (m/z) are transmitted to the detector.
Ionized proteins are accelerated in an electrical field and their
mass-to-charge ratio measured.
In a perfect dendrimer structure each -N[H.sub.2] group "carries" roughly 200 Da of mass and it is obvious that because of increased
mass-to-charge ratio the cyclic product should have lower electrophoretic mobilities.
analyzer detection, ionization of protein molecules, and
mass-to-charge ratio separation.
MS enables identification of the composition of a compound based on its
mass-to-charge ratio. A sample is ionized, and the charged molecular particles are propelled through an electromagnetic or electric field for separation by differences in mass (molecular weight).
The concept of mass spectrometry consists of fragmenting molecules and creating ions in a gas phase under high vacuum, and then separating the fragments according to their
mass-to-charge ratio. The technique requires a very small amount of sample, can elucidate structural information and determine molecular weight.
These peptides can be quantified by use of selected reaction monitoring, which specifically selects the
mass-to-charge ratio (m/z) of an enzyme-generated peptide and then detects a specific fragment ion of the peptide generated within the mass spectrometer.
In MRM mode (1), the respective observed T2 [[M + 2H].sup.2+] ions,
mass-to-charge ratio (m/z) [beta], 466.8, and [delta], 480.3, were selected and fragmented, and the most informative singly-charged peptide fragments VTALWGK, 675.4, and VNALWGK, 688.4, targeted.
(3) identified peaks at an initial
mass-to-charge ratio (m/z) and matched peaks by considering peaks within [+ or -] 0.2% of molecular weight to be the same peak.