[??] (970), normalized absorbance
= A (970)/A (2,236) and [??] (723) = A (723)/A (2,236).
The [DELTA][A.sub.450] was measured as the difference in absorbance
between the bilirubin peak at 450 nm, and a baseline was established between the absorbance
of wavelengths flanking the bilirubin peak (~370 run and 525 nm) on a linear scale.
To identify the wavelength at which absorbance
can reflect the methyl groups (-[CH.sub.3]), a polypropylene (PP) spectrum was measured at the same wavelength range.
The sample was eluted from the column with phosphate buffer pH 6.65 at a flow rate of 0.5 ml [min.sup.-1] and the absorbance
of the protein was monitored using a multi-wavelength detector (Model 490E; Millipore-Waters, Australia).
The difference is absorbance
. Virtually all molecular entities selectively absorb light energy as particular frequencies in the NIR range.
measurements were performed with a Perkin Elmer Lambda 850 spectrometer equipped with a 150 mm integrating sphere (IS) detector.
Where, Ac is absorbance
of control, As is the absorbance
(1) reminds us of the difficulty of assessing amniotic fluid bilirubin absorbance
in specimens contaminated by blood.
Relative allyl absorbances
were determined directly after vulcanization of the compound and are presented in Fig.
To record absorbance
, UV-visible spectrophotometer UVD-3200 Labomed,
Method B is based on dual wavelength spectrophotometry where ASC can be determined by difference in absorbance
at 249.8 and 285.8 nm.