The GNAT family consists of at least 12 enzymes with different cellular functions that acetylate
both histone and nonhistone proteins.
pCAF assists in remodeling the chromatin structure of the MHC II promoter where it acetylates
histones H3 and H4  and also regulates CIITA's nuclear relocation by acetylating CIITA K141 and K144, leading to increased activation of CIITA and increased expression of MHC II .
In order to perform more sophisticated ALMS experiments, including protein digestions, it is imperative to eliminate any excess anhydride so as not to inadvertently acetylate
amino acids becoming accessible as a result of processes like enzymatic digestions.
A decade ago, histone acetyl transferases (HATs) were first reported to acetylate
the tumour suppressor p53, leading to the notion that HATs and HDACs are not just for histones.
It has been previously documented that E2F1 is able to recruit other histone acetyltransferases such as GCN5 to acetylate
localized H3K9 residues (Guo et al.
Persistent work carried out in our laboratory led to the discovery of a microsomal enzyme which had the ability to acetylate
certain functional proteins utilizing polyphenolic acetates (PA) as the acetyl group donor [11-14].