Morphological changes, such as nuclear and chromatin condensation and fragmentation, that occur in apoptosis can be assessed by fluorescent microscopy using supravital dyes such as acridine
orange (AO) and ethidium bromide (EB).
Figure 2 shows that microbial counts in the soil column determined by acridine
orange direct counting (AODC) methods did not significantly change 1 week after flushing with ethanol compared with counts in soils before flushing (8.63 x 107 [+ or -] 2.22 x [10.sup.7] cells/g soil and 6.87 x [10.sup.7] [+ or -] 8.9l x [10.sup.6] cells/g soil, respectively).
Pattern of DNA denaturation was evaluated using fluorescent test of acridine
orange is intercalated into the nucleic acid of cells.
For analysis, two straws per replicate were thawed at 37AdegC, pooled and processed for acridine
A solution containing 100 [micro]g/mL Acridine
Orange and 100 [micro]g/mL Ethidium Bromide was prepared as previously described and added to the slides containing promastigotes [34, 35].
Morphological observation of DAPI and acridine
orange/propodium iodide staining documented typical characteristics of apoptotic cell death.
For CLSM, the 24 h treated root samples were stained with acridine
orange for 10 min and images was acquired as mentioned in CLSM of coverslip biofilms.
LMP was evaluated by acridine
orange (AO), a lysosomotropic base containing metachromatic fluorophore, which becomes charged and retained within acidic compartments .
Koehn, Dercitin, a new biologically active acridine
alkaloid from a deep water marine sponge, Dercitus sp., JACS, 110, 4856 (1988).
Sperm DNA integrity was assessed using acridine
orange assay (Martins et al.
Clearing allowed imaging with excellent cellular and nuclear resolution of SYTOX Green or acridine
orange-stained specimens more than 500 [micro]m deep into formalin-fixed human prostate, liver, breast, and kidney samples (Figures 1, a through d; 2, a through d).