For analysis, two straws per replicate were thawed at 37AdegC, pooled and processed for acridine
orange/ethidium bromide (AO/EB) staining assay was used to identify apoptotic and necrotic cells.
Differential Staining of DNA and RNA in intact cells and isolated cell nuclei with acridine
orange" Methods in Cell Biology 33: 285 - 98 (1990)
Darkfield microscopy or staining with acridine
orange might be required to increase sensitivity.
Depending on the acidity of AVO, acridine
orange staining assay (orange: low pH condition ([less than or equal to] 3.
Morphological observation of DAPI and acridine
orange/propodium iodide staining documented typical characteristics of apoptotic cell death.
A test for the practical evaluation of male fertility by acridine
orange (AO) fluorescence.
For CLSM, the 24 h treated root samples were stained with acridine
orange for 10 min and images was acquired as mentioned in CLSM of coverslip biofilms.
Orange- (AO-) Propidium Iodide (PI) Staining.
The chromatin structure of the spermatozoa was evaluated using acridine
orange according to a methodology proposed by Bochenek et al.
Damage by visible light to the acridine
orange-DNA complex" Biophys.
Orange Staining for Determination of Apoptosis in Zebrafish Embryos