acridine orange


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acridine orange

[′ak·rə‚dēn ′är·inj]
(organic chemistry)
A dye with an affinity for nucleic acids; the complexes of nucleic acid and dye fluoresce orange with RNA and green with DNA when observed in the fluorescence microscope.
References in periodicals archive ?
Micronucleus is an excellent genotoxic biomarker [15, 20]; therefore, the staining technique with acridine orange helps differentiate micronucleated cells.
3 shows the morphology of U937, HL60 and K562 cells treated with ME and EAF and stained with acridine orange and ethidium bromide.
Abnormal nuclei as purple-violet sperm heads have been shown to be correlated with results by the acridine orange method.
The nuclear staining from the acridine orange created the necessary contrast between the nuclear and cytoplasmic areas of the cell, thereby aiding in the recognition of the tissue architecture.
Besides conventional diagnostic approaches, fluorescent staining technique was implemented to diagnose blood parasites (Tierney et al., 1983) in fast and reliable way using stains like Acridine orange. Hansen et al.
Ten [micro]L of lymphocyte suspension on the microscopic glass was mixed with 10 [micro]L of acridine orange (10 mg/mL)/ethidium bromide solution (10 mg/mL), covered by cover slip and analyzed under the epifluorescence microscope (BX 50, Olympus, Tokyo, Japan) using 400x magnification.
Diagnostic Accuracy of the acridine orange fluorescence staining and papanicolaou staining in detection of oral cancer.
Autophagy is also characterized by acidic vesicular organelle (AVO) formation, which can be assayed by acridine orange staining [17].
After 7 h, RPE cell death was monitored by propidium iodide (PI) using FACS analysis and by ethidium bromide and acridine orange fluorescent staining.