Shallow wounds were treated with Wound-Kote (48% isopropyl alcohol, 0.04% Acriflavin
), and deeper lacerations were cleaned with isopropyl alcohol but left open to avoid closing in infections.
This result was interpreted in Osmanthus (in conjuction with the results of acriflavin
staining) as indicating the presence of lignin in the torus.
Others proposed indications are: daily washing of the ulcerate wounds with 3% oxygenated water, submerging in Burrow solution at 1:20/tid for 15 minutes or apply a triple watery coloring (brilliant green 1:400, genciana violet 1:400 and acriflavin
1:1000) three times a week, as in the case of crotale bites.
Before transferring, 50 [micro]L/10 mL 0.5% acriflavin
, 40 [micro]L/10 mL 0.5% nalidixic acid in 0.1 M NaOH, and 0.1 mL 5% ferric ammonium citrate were added to the Fraser broth.
Wound was cleaned with Hydrogen peroxide solution, 5% Acriflavin
solution and 7% Tincture iodine solution routinely.