Effect of sulfated glycoconjugates on capacitation and the acrosome reaction
of bovine and hamster spermatozoa.
This agglutination is likely due to spontaneous acrosome reactions
which could have occurred at some point during freezing or thawing.
A stimulatory effect of methyl xanthine on capacitation and acrosome reaction
has also been demonstrated (Aitken et al., 1993).
Ram spermatozoa cocultured with epithelial cell monolayers: an in vitro model for the study of capacitation and the acrosome reaction
. Mol Reprod Dev.
The reason for this is a membrane protective capacity of still unknown egg yolk components (Witte et al., 2009), but the low-density lipoprotein fraction (LDF) of egg yolk (Awad and Graham, 2002) may interact with the plasma membrane of sperm and also decrease the efflux of cholesterol and phospholipids from the sperm membrane and thus prevent premature capacitation and subsequent acrosome reaction
(reviewed by Purdy, 2006).
Contact with the vitelline layer overlying the microvilli initiates the typical acrosome reaction
, in which the outer acrosomal membrane and plasma membrane fuse at the apex and roll back in a manner that releases the contents of the acrosomal vesicle onto the vitelline layer [ILLUSTRATION FOR FIGURES 25, 26 OMITTED].
Indeed, SA certainly does not evaluate many of the parameters of sperm that may impact MFI such as an intact acrosome reaction
These are suitable conditions for sperm cell capacitation 10 and also to evaluate their ability to undergo acrosome reaction
. The latter was induced by incubating 5x106 sperm cells with 20 [micro]L of perivitelline layer (PVL) and kept for 40 min at 37 [degrees]C (Ahammad et al., 2011b).
Mouse gamete interactions during fertilization in vitro: chlortetracycline as a fluorescent probe for the mouse sperm acrosome reaction
. J Cell Biol 83: 544-555.
Human sperm CRISP2 is released from the acrosome during the acrosome reaction
and re-associates at the equatorial segment.
Sperm capacitation and the acrosome reaction
. Hum Reprod 1998;13:9-19.
Genome-wide association study implicates testis-sperm specific fkbp6 as a susceptibility locus for impaired acrosome reaction