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Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskalea.
Under the terms of the planned transaction, Euroscreen Products would transfer its portfolio of GPCR screening tools and its exclusive global license to aequorin technology, granted by the University of Georgia Research Foundation Inc., to PerkinElmer.
Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium.
Some of the words Pete has played include AEQUORIN (a protein that some jellyfish emit so that it looks as if they are glowing), BEATNIK, VERITAS and HEADRIGS (specialised tools used to turn logs into planks).
Four decades later, Shimomura, now at the Marine Biological Laboratory in Woods Hole, Mass., and his colleagues have finally teased out the crystal structure of this photoprotein, known as aequorin.
The discovery of aequorin and green fluorescent protein.
New developments for the FLIPR Tetra will include modifications for use with the aequorin luminescence assay, an assay's whose popularity for high-throughput screens is growing.
Using the [Ca.sup.2+]-sensitive luminescent reporter, aequorin, and a custom-designed photon imaging microscope (PIM), we have started to re-investigate the patterns and possible functions of the blastula period [Ca.sup.2+] transients.
By loading embryos with the [Ca.sup.2+]-sensitive luminescent reporter, aequorin, and using a custom-designed photon-imaging microscope to continually visualize the [Ca.sup.2+] signaling patterns, we are currently generating spatial and temporal maps of the [Ca.sup.2+] signals that occur during pronephric formation in these two intact model systems, as well as in animal caps.
He discovered green fluorescent protein and aequorin, biological markers that are now staples in the biologist's toolbox.
Aequorin, from the jellyfish Aequorea aequorea, was the first calcium-sensitive photoprotein discovered by us in 1961.
"The bioluminescent jellyfish Aequorea emits 'green' light in vivo, whereas the pure photoprotein aequorin extracted from the same organism emits 'blue' light on addition of [Ca.sup.2+]." Osamu Shimomura made this observation and identified a green fluorescing molecule in 1962; then reported its purification and characterization in 1974 from 30,000 specimens of the hydrozoan jellyfish.
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