According to the company, when used in mAb purification, this combination allows the hybrid purifier to provide superior product purity before the protein A affinity column
, which in turn results in even greater purity downstream of the protein A operation.
When used in mAb purification, this combination of attributes allows the hybrid purifier to provide superior product purity before the protein A affinity column
, which in turn results in even greater purity downstream of the protein A operation, according to the company.
t] value of a given affinity column
is obtained by a concentration dependence analysis by using an appropriate analyte, [K.
The spiked sample is then injected onto the workstation where an affinity column
pulls the immune complex out of solution.
The immune affinity column
could purify the recombinant Fusion GST-SK to homogeneity as a single band of about 71 kD on SDS-PAGE (Figure 5).
chartarum similarly revealed lysine-, cysteine-, and histidine-SG adducts after exhaustive digestion, affinity column
enrichment, and MS analysis.
The 75 kDa GST-tagged protein was purified on a GST affinity column
and used in pull-down experiments with either clarified squid brain homogenates or FLAG-tagged Rab 11a transfected HEK cell lysates.
The serum IgG was purified by ammonium sulfate precipitation followed by protein A affinity column
The American Organization of Agricultural Chemists, for example, recently approved a method that uses an antibody to bind aflatoxin to a matrix in an affinity column
, then removes it from the antibody and quantifies, using high-pressure liquid chromatography.
The affinity column
was regenerated by washing with 5 mL of ultrapure water and 5 mL of 0.
americana that reacted to MAb38G6 that had previously been shown to be an allergen was prepared from the CR crude extract using MAb affinity column
chromatography as previously described (Sookrung et al.
As a complementary method for isolating myosin-V binding partners, an affinity column
was generated by attaching GST-GTD to a GSTrap column.