affinity chromatography

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affinity chromatography

[ə′fin·əd·ē ‚krō·mə′täg·rə·fē]
(analytical chemistry)
A chromatographic technique that utilizes the ability of biological molecules to bend to certain ligands specifically and reversibly; used in protein biochemistry.
References in periodicals archive ?
The affinity column was loaded with ~1.2 L of the LNCap cell line with 0.990 mg/L PSA.
Earlier purification schemes for Trx and TrxR involved anion exchange and affinity column chromatography steps.
In this study, PE, PG, and PL were separated from CPEusing an HM-CL-AIS affinity column. The separated enzymes were added to orange for comparing the effects of separation on physicochemical properties during the winemaking process and the decrease of methanol content in wine.
A complementary approach that can be used is to see how the retention of a solute or group of solutes is altered as a change is made in the structure of a binding agent in an affinity column. For instance, this method has been employed in HPAC to look at how modifying specific amino acids (e.g., Cys-34 or Trp-214) on HSA affects the ability of this protein to bind to various drugs (55-58).
In the isolation of the commercial seeds and flour proteins analyzed, the affinity column with resin Sepharose CNBr 4B (Sigma, St Louis, United States) trypsin derivative was used, pre-equilibrated with buffer Tris-HCl 0.05 mol.
They have developed the first aptamer-based affinity column for the concentration and decontamination of OTA.
According to the company, when used in mAb purification, this combination allows the hybrid purifier to provide superior product purity before the protein A affinity column, which in turn results in even greater purity downstream of the protein A operation.
When used in mAb purification, this combination of attributes allows the hybrid purifier to provide superior product purity before the protein A affinity column, which in turn results in even greater purity downstream of the protein A operation, according to the company.
Therefore, once the [B.sub.t] value of a given affinity column is obtained by a concentration dependence analysis by using an appropriate analyte, [K.sub.d] values for other analytes can be determined by only one chromatographic run for each, provided that their concentrations are adequately low (e.g., lower than 1% of [K.sub.d]).
The spiked sample is then injected onto the workstation where an affinity column pulls the immune complex out of solution.
The fully functional Atl protein was purified using a nickel affinity column and can facilitate structural and biochemical studies.
Chromatography on 2, 5' ADP Sepharose 4B Affinity Column. The Sephacryl S-300 concentrated fractions containing the G6PD activity were applied onto a 27, 57 ADP Sepharose 4B affinity column (3 cm x 2.4 cm i.d.) equilibrated with 0.02 M Tris/HCI buffer, pH 7.6, containing 1mM [beta]-mercaptoethanol and 1mM EDTA (Buffer A).