Effects of Complement Inhibition With Eculizumab Observed in Patients with Atypical Hemolytic Uremic Syndrome (aHUS) and Cold Agglutinin
Vicia faba agglutinin
, the lectin present in broad beans, stimulates differentiation of undifferentiated colon cancer cells.
We prepared the multilectin column by mixing equal amounts of agarose-bound concanavalin A, agarose-bound wheat germ agglutinin
, and agarose-bound Jacalin in an empty PD-10 disposable column (GE Healthcare).
In comparison to splenocytes and thymocytes proliferation of Abrus agglutinin
and abrin (Ramnath et al.
Transferrin and apoC-III both showed abnormal IEF patterns, and peanut agglutinin
lectin staining was increased in the fibroblasts.
In vitro influence of Phaseolus vulgaris, Griffonia simplicifolia, concanavalin A, wheat germ, and peanut agglutinins
on HCT-15, LoVo, and SW837 human colorectal cancer cell growth.
Though we have not observed agglutinin
properties of nebrodedolysin, there are some other proteins containing both agglutinin
properties and hemolytic activity, such as Laetiporus sulphureus lectin (LSL).
The most successful approach has been based on the difference in the binding affinities of the AFP glycoforms to various lectins, particularly Lens culinaris agglutinin
and concanavalin A (13-16).
Urtica dioica agglutinin
(UDA), a lectin from stinging nettle roots, was reported to directly inhibit cell proliferation of Hela cells and block the binding of EGF to its receptor, and was regarded to be responsible for inhibiting effects in BPH treatment (Wagner et al.
Briefly, 2 [micro]L of serum was electrophoresed on a Lens culinaris agglutinin
agarose gel and then blotted on a mouse anti-AFP-coated nitrocellulose membrane.
The effect was more pronounced than with wheat germ agglutinin
, which possesses the same sugar specificity and the mannose-specific agglutinin
Sepharose columns) Lectin affinity chromatography CDT-TJA (separated on T.