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3 that the peaks al about 204 ppm are attributed to carbon atoms in aldehyde groups, further confirming the presence of aldehyde in the resin, (29), (30) and the relative peak area increases on increasing the amount of isobulyraldehyde.
In addition, the UIF resins also contained hydroxyl groups, and the content of aldehyde groups in the resin increased with the increase of isobutyraldehyde.
This conclusion can be made from the NMR spectra of the formed resins, where no amino and aldehyde groups can be seen, and from the hydroxyl equivalent of formed resins (Table 2), which increases with increasing molar ratio.
3), which means an increase of the quantity of aldehyde groups in individual reaction mixtures.
5, the ratio between amino and aldehyde groups is 2:2.
At the beginning of the reaction the aldehyde groups react more intensively with amino groups than with imino groups.
The aldehyde groups derived from the agarose film were covalently linked with amino groups of antigens.
Activated agarose provided a thin layer on the glass with active aldehyde groups which can bind to amino groups on various biomolecules, such as DNA, proteins, peptides and phospholipids.
Previous studies ascertained active aldehyde groups in agarose could be linked to amino groups in biomolecules and after UV crosslinking, the covalent binds could be formed.
OXY-Cel contains aldehyde groups introduced directly into the cellulose chain without any spacer.

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