The aliquotter can be pro- grammed for aliquot
volumes in secondary tubes ranging from 300 [micro]l to 9,999 [micro]l, processing up to 400 samples per hour while checking for clots.
It took ~20 min to perform triplicate measurements of all 3 aliquots
from an individual patient.
The extension requires two additional steps to the traditional transformation protocol: to allow the students time to treat their DNA sample and then to have more plates available for the students to transform the appropriate number of aliquots
of competent cells (usually six: no DNA negative control, positive control of plasma with no treatment, amylase, protease, DNase, and RNase treatment).
Triple observations were performed for each aliquot
. Results were expressed as the cellular location in which fluorescence was observed for each experimental group: acrosome (A), equatorial band (E), postacrosome (P) and all over the head (H); and as a three-category ordinal scale for fluorescence intensity (low, medium and high intensity).
CONCLUSION: When comparing Charter Medical multiple aliquot
bag sets and the Charter Medical syringe aliquot
system to provide neonatal transfusions, the use of the syringe system decreased blood waste and proved more cost effective.
of VMS is used that has an amount of suppressor necessary to inhibit the deposition to the maximum possible extent, along with a corresponding amount of brightener (leveller intercept solution).
of each sample were treated with 10% trichloroacetic acid and sedimented at 1,000 x g for 15 min to precipitate macromolecules.
Rodby and Firanek (2003) compared the batch and aliquot
methods and decided that the aliquot
method was easier because there was less physical lifting, mixing and discarding of effluent.
John's wort were the most active single-entity herbal teas with aliquots
of extracts producing inhibitions >70% against three or more iso-form mediated-metabolisms (Table 4).
As the powder dissolves, a 20 mL aliquot
is withdrawn from the solution every 30 seconds for the duration of the 45-minute experiment.
This is prepared by injecting 2 mL of 0.9% saline into a 100-U vial of botulinum toxin, then drawing up a 0.4-mL aliquot
containing 20 U into a 1-mL insulin syringe.
Thus the measuring procedure had to be improved so that all the changes of sensitivity would be considered and only one aliquot
used (Duller 1995).