The cells were resuspended in cold HBSS and aliquoted
in 20000 cells per 16.4 [micro]L.
Thereafter, culture supernatants were aspirated, centrifuged at 15500 g for 5 min at 4[degrees]C, aliquoted
, and stored at -70[degrees]C until quantification of cytokines.
Pooled stock from fresh samples from healthy psittaciform birds was prepared, aliquoted
, and stored for use.
The crude antigen obtained was aliquoted
and stored at -70[degrees]C until use.
Solutions were diluted in human fresh-frozen plasma, aliquoted
(100 [micro]L), and lyophilized.
Pellet containing purified Anaplasma bodies was resuspended in TS-PI Buffer, aliquoted
and preserved under liquid nitrogen.
Supernatants were filter-sterilized through a 0.22[micro]m filter, aliquoted
and stored at -20[degrees]C until use.
the cell suspension (100 pL) into each well of two 96-well tissue culture plates and incubated them at 37[degrees]C.
Bicuculline was aliquoted
in distilled water and diluted (1000-fold) in Ringer's to 10 [micro]M
Eggs were aliquoted
with a pasteur pipette to different treatments and to a control dish of eggs without added sperm as fertilization controls.
Serum samples were aliquoted
and kept at -20oC until analysed.
Serum or plasma samples were thawed and aliquoted
into single-use vials for DMF immunoassays.