From each sample, 140 [micro]L was aliquoted
for nucleic acid extraction.
Pooled stock from fresh samples from healthy psittaciform birds was prepared, aliquoted
, and stored for use.
The plasma layers were aliquoted
into vials and refrigerated for transport to Quest Diagnostics (Teterboro.
22[micro]m filter, aliquoted
and stored at -20[degrees]C until use.
These S-9 (supernatant 9000Xg) preparations were then aliquoted
into five test tubes that contained a prepared reaction mixture that served as a Metabolic Activation System (MAS).
Transfected DCs are matured, aliquoted
Samples were aliquoted
for sample-stability studies at refrigerated (4[degrees]C), ambient (22[degrees]C), and frozen (-20[degrees]C) temperatures, as well as for freeze/ thaw experiments.
3 mL of plasma was aliquoted
from each sample and frozen at -20[degrees]C overnight (~22 hours), and the remaining plasma was stored on cells and analyzed after 1, 3, and 5 hours of storage at room temperature.
Each tube is assigned a route from the automation system's software and is centrifuged, decapped, aliquoted
, recapped, or sorted, depending on the tests requested.
A stock solution of clavulanic acid (2000 [micro]g/ml) was prepared, aliquoted
into small vials and stored at -20[degrees]C.
Blood was obtained from each pig by cardiac puncture and directly aliquoted
into 2-ml sterile vials, one aliquot used liquid sodium heparin (1,000 USP units/ml) as an anticoagulant to obtain the whole blood for lymphocyte tests, another allowed to clot for 4 h for serum preparation.
The remaining swab transport medium was filtered, and 100 [micro]L of filtrate was aliquoted
onto each of 3 tissue culture plates containing rhabdomyosarcoma, Vero, and 293T cell lines for virus isolation.