Doherty, "Synovial fluid calcium pyrophosphate dihydrate crystals and
alizarin red positivity: analysis of3000 samples," British Journal of Rheumatology, vol.
Production of external fluorescent marks on Golden Perch fingerlings through osmotic induction marking with
Alizarin Red S.
In this study we successfully isolated MSCs from bone marrow of the mature rats, and showed their ostegenic potential and forming calcification tissue detected by
Alizarin Red staining.
To assess late stage of osteogenesis,
Alizarin Red staining was performed quantitatively.
Dual staining of the corneal endothelium with vital dyes using 0.25% trypan blue and 0.2%
alizarin red is the most common method for determining viable and nonviable cells in areas where cells cannot be identified using specular microscopy (SPERLING, 1977; TAYLOR & HUNT, 1981).
To further confirm the bone formation induced by fluoride in osteogenic medium,
Alizarin red S staining was used to analyze mineralization in PDLCs in vitro.
Alizarin red staining showed bone-nodule formation in the presence of the osteogenic medium.
To confirm osteogenesis and adipogenesis,
Alizarin Red and Oil Red O staining were applied, respectively.
Alizarin red, calcein and tetracycline are commonly used PFCs in bone histomorphometry, appearing as red, green and bluish green respectively under the fluorescent microscope.
After fixation, the specimens were processed for staining with 40 mM
alizarin red S, which stains areas rich in nascent calcium.
In future studies,
Alizarin Red and/or Von Kossa Staining will be performed on cell culture samples in order to visualize mineralization.
Differentiation was assessed by
alizarin red staining.