Doherty, "Synovial fluid calcium pyrophosphate dihydrate crystals and alizarin red
positivity: analysis of3000 samples," British Journal of Rheumatology, vol.
Production of external fluorescent marks on Golden Perch fingerlings through osmotic induction marking with Alizarin Red
In this study we successfully isolated MSCs from bone marrow of the mature rats, and showed their ostegenic potential and forming calcification tissue detected by Alizarin Red
To assess late stage of osteogenesis, Alizarin Red
staining was performed quantitatively.
Dual staining of the corneal endothelium with vital dyes using 0.25% trypan blue and 0.2% alizarin red
is the most common method for determining viable and nonviable cells in areas where cells cannot be identified using specular microscopy (SPERLING, 1977; TAYLOR & HUNT, 1981).
To further confirm the bone formation induced by fluoride in osteogenic medium, Alizarin red
S staining was used to analyze mineralization in PDLCs in vitro.
staining showed bone-nodule formation in the presence of the osteogenic medium.
To confirm osteogenesis and adipogenesis, Alizarin Red
and Oil Red O staining were applied, respectively.
, calcein and tetracycline are commonly used PFCs in bone histomorphometry, appearing as red, green and bluish green respectively under the fluorescent microscope.
After fixation, the specimens were processed for staining with 40 mM alizarin red
S, which stains areas rich in nascent calcium.
In future studies, Alizarin Red
and/or Von Kossa Staining will be performed on cell culture samples in order to visualize mineralization.
Differentiation was assessed by alizarin red