One of the studies performed in Caucasians showed 6% mutation in allelic frequency
of HFE gene of C282Y variant with or without symptoms of iron over load, results of this study also varied due to multi centric, long period and large number of participant22.
The allelic frequency
was estimated by the percentage of polymorphism (P) by the formula:
SNP selection was based on a review of literature, allelic frequency
, and functional position.
The result of the current study was showed that most patients were homozygous carry the mutant (A) alleles whereas the control individuals were have the (G) wild whereas origin GC type alleles dependent on the allelic frequency
and the odd ratio results.
DiaCarta said the analytical sensitivity ranges from 0.1 to 0.5% for variant allelic frequency
, providing lower detection limit compared with competition due to application of DiaCarta's proprietary XNA technology.
The allelic frequency
of CYP3A4*1B changes among different ethnic groups; CYP3A4*1B allelic frequency
is dominant in black subjects with a range of 38.4 to 82.4% (Table 3).
A reduction in the allelic frequency
of the T (minor) allele is observed in the patient population.
Low molecular weight glutenin subunits (LMW-GS) variation: Distribution of allelic combination of LMW-GS at Glu-A3 and Glu-B3 is given in Table 4, while Table 5 is showing allelic frequency
for Glu-A3 and Glu-B3 loci.
The genetic diversity of adult and sub-adult individuals was estimated based on the total number of alleles (k), on the highest allelic frequency
(Fa), on the observed ([H.sub.o]) and expected (He) heterozygosity (at Hardy-Weinberg equilibrium in each locus and across all loci), and on the polymorphism information content (PIC), in order to verify the quality of the used loci.
Because rs12688220 resides at X chromosome, we analyze genotype separately by gender (3 x 2 in female and 2 x 2 in male groups) and analyze allelic frequency
of both genders (2 x 2) .
Genotypic and allelic frequency
of the IL-6 (-572G/C) polymorphism in patients with CD and healthy controls Genotypes Patients Healthy p OR (95% Cl) with CD, controls, (n=84) % (n=83) % CC 38 (45.2) 52 (62.7) References CG 36 (42.9) 29 (34.9) p: 0.294 1.39 (0.748-2.609) GG 10 (11.9) 2 (2.4) p: 0.018 5.47 (1.161-25,800) C 112 (66.6) 133 (80.1) References G 56 (33.4) 33 (19.9) p: 0.070 0.24 (0.050-1.224) CD: celiac disease; OR: odds ratio; Cl: confidence interval Table 2.
As no heterozygous individuals were found so allelic frequency
and genotypic frequency was same.