The data obtained from recipients included the most recent creatinine level (mg/dl), peak creatinine (mg/dl), viral load of polyomavirus (DNA copies/ml), and the quantization of alloantibodies
, pre- and post-transplant.
 Consequent to the introduction of routine RhIG immunoprophylaxis, alloantibodies
other than anti D have emerged as an important cause of HDFN and now responsible for greater proportion of these cases.
When patients develop rare or multiple alloantibodies
, molecular techniques, such as genotyping, may be required.
Pretransfusion blood samples from all SCD patients were tested for the presence of alloantibodies
by gel technology using commercial 3-cell panel (Diacell, Diamed ID, Switzerland), with polyspecific (anti-IgG + C3d) anti-human globulin (AHG) and homozygous expression of the antigens.
recognize antigenic epitopes displayed by HLA molecules on the transplanted allograft through complement-dependent and independent pathways .
The technique: Lymphocytes are tested with a panel of sera containing well-characterized HLA-specific alloantibodies
. Each serum is placed in a microtiter well of a Terasaki plate (60-72 wells/plate).
and the mechanism of the antiglobulin-augmented lymphocytotoxicity procedure.
However, although all canine blood group antigens can stimulate the formation of alloantibodies
, DEA 1 seems to be the most immunogenic and also is considered the most clinically important.
Hendrickson, "Clinically significant anti-KEL RBC alloantibodies
are transferred by breast milk in a murine model," Vox Sanguinis, vol.
Our data may explain previously published data, where MSC-[gamma] infused mice had higher levels of circulating anti-donor IgM and IgG alloantibodies
, which resulted in the rapid induction of antibody-mediated rejection .
Blumenberg, "Management of bleeding in a multi-transfused patient with positive HLA class I alloantibodies
and thrombocytopenia associated with platelet dysfunction refractory to transfusion of cross-matched platelets," Blood Coagulation and Fibrinolysis, vol.