The results of InterPro v56.0 and Superfamily v1.75 showed that Der f 33 belonged to the alpha-tubulin
protein family (InterPro No.
Identified protein Cytoskeletal protein 17 gi|3642627 Alpha-tubulin
, partial 18 gi|6678469 Tubulin alpha-1C chain 19 gi|49868 Put.
Western blot analysis of the protein indicates an increased expression of protein from days 0 to 90 d whereas expression of acetylated alpha-tubulin
was observed only in d60 and d90 rat testis (Figures 1(b) and 1(c)).
Zhou, "Expression of alpha-tubulin
and MDR1 and their correlation with the biological features of non-small cell lung carcinoma," Chinese Journal of Oncology, vol.
Here to best assess microtubules organization in myotubes exposed to 50 [micro]M CisPt and the impact on autophagy, we analysed alpha-tubulin
distribution by immunofluorescence in treated or control muscle cells at 4 h.
The nitrocellulose membranes were incubated overnight at 4[degrees]C with antibodies against TLR4 (toll-like receptor 4), and nuclear factor kappa B p50 (NF-kBp50), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI58), perilipin, AKT, PKC, AMPK, glutathione peroxidase, catalase, and alpha-tubulin
were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA) diluted in blocking buffer to which 1% bovine serum album (BSA) was added and then washed for 30 min in blocking buffer without BSA.
Tubulin molecules occur as dimers containing one alpha-tubulin
(1TUB_A) and one beta-tubulin (1TUB_B) molecule.
Monoclonal antibodies specific for an acetylated form of alpha-tubulin
recognize the antigen in cilia and flagella from a variety of organisms.
To normalize the total amount of cDNA in each reaction, cotton alpha-tubulin
gene was co-amplified as the internal control.
Name of Corresponding Homologous gene after BLAST searching cDNA clone genomic clone (% nucleotide sequence identity) STUB1 9-1 Hordeum vulgare alpha-tubulin
3 (90%) accession number AJ132399 SPRP1 10-1,14-1 Zea mays proline-rich protein (84%) accession number Y17332 SAQ1 16-1 Zea mays plasma membrane MIP protein (pip2) (96%) accession number AF131201 SEF1[alpha] 19-1 Zea mays Elongation factor 1[alpha] (93%) accession number U76259 ACKNOWLEDGMENTS