Good examples of vitamin antagonists include isonicotinic acid hydrazide (antagonist of pyridoxine), aminopterin
(folate antagonist), dicumarol (vitamin K antagonist), and avidin (interferes with biotin absorption).
wheat gluten contaminated with aminopterin
, a toxin used in some countries as rat poison.
The substance in the food was identified as aminopterin
, a cancer drug that once was used to induce abortions in the United States and is still used to kill rats in some other countries, said Patrick Hooker, state agriculture commissioner in New York, where scientists are investigating the tainted pet food.
In utero exposure to aminopterin
, an antagonist of folic acid, has been associated with anencephaly, meningocele, hydrocephalus, and cleft lip and palate (Thiersch 1952; Warkany et al.
The fused cells were cultured in 96-well plates in DMEM (Invitrogen) containing 200 mL/L fetal calf serum, 200 mmol/L glutamine, 10 g/L OPI (oxaloacetic acid, pyruvic acid, insulin), and 20 g/L HAT (hypoxanthine, aminopterin
, thymidine; Sigma) at 37 [degrees]C in a 5% C[O.
Abstract #2052: "Pharmacokinetics of PT-523, A Novel Aminopterin
Analogue, in Patients with Solid Tumors.
Hybrid cells were cultured in medium containing aminopterin
, hypoxanthine, and thymidine (HAT medium) with 100 mL/L fetal calf serum.
Talotrexin is a novel, non-classical antifolate that is a water-soluble, non-polyglutamatable analogue of aminopterin
Optimem 1 with Glutamax-1 and HAT supplement (hypoxanthine, aminopterin
, and thymidine) were products of Life Technologies.
Discontinuation of the development of aminopterin
The fused cells were cultured with 96-well culture plates (Nunc) in RPMI 1640 (Nissui) containing fetal bovine serum, hypoxanthine, aminopterin
, and thymidine.