ammonium sulfate fractionation

ammonium sulfate fractionation

[ə¦mōn·ē·əm ¦səl‚fāt ‚frak·shə′nā·shən]
(biochemisrty)
A protein purification technique that utilizes the varying precipitation rates of different proteins in ammonium sulfate to obtain considerable separation.
References in periodicals archive ?
The extracellular cellulase produced, using 5 liters medium was subjected to ammonium sulfate fractionation. An attempt was made to separate cellulase components by binding with DEAE Cellulose' as anion exchanger.
Thus over all two samples Sample A and Sample B were obtained which were subjected to ammonium sulfate fractionation and processed further.
As a result of ammonium sulfate fractionation of Sample A and Sample B, two fractions were obtained from each at 60% saturation and 80% saturation respectively.
Table: 1 Progress of ammonium sulfate fractionation of cellulose of T.
This formed an adequate basis of bulking the growth samples up to 8th day as sample A and those from to 9th to 21st as sample B, which were subjected to ammonium sulfate fractionation and it's binding with DEAE cellulose.
Ammonium sulfate fractionation of the extract indicated the presence of toxicity toward P.
After repeated ammonium sulfate fractionation, the sample was loaded on to the hydrophobic column (Resource PHE), and it eluted as a single peak (Fig.1).
r-hCK-MB was purified from crude extract by ammonium sulfate fractionation, followed by hydrophobic, anion-exchange, and gel-filtration chromatography (Table 2).