The extracellular cellulase produced, using 5 liters medium was subjected to ammonium sulfate fractionation. An attempt was made to separate cellulase components by binding with DEAE Cellulose' as anion exchanger.
Thus over all two samples Sample A and Sample B were obtained which were subjected to ammonium sulfate fractionation and processed further.
As a result of ammonium sulfate fractionation of Sample A and Sample B, two fractions were obtained from each at 60% saturation and 80% saturation respectively.
Table: 1 Progress of ammonium sulfate fractionation of cellulose of T.
This formed an adequate basis of bulking the growth samples up to 8th day as sample A and those from to 9th to 21st as sample B, which were subjected to ammonium sulfate fractionation and it's binding with DEAE cellulose.