Using the same procedure, solid ammonium sulfate was added to the supernatant to obtain, sequentially, 20% increments of ammonium sulfate fractionation.
Ammonium sulfate fractionation of the extract indicated the presence of toxicity toward P.
herreni are most likely proteins or peptides since they were thermo-labile and were precipitated by ammonium sulfate fractionation, and had molecular weights greater than 3.
r-hCK-MB was purified from cell lysate by three chromatographic methods (hydrophobic, ion-exchange, and gel-filtration) after ammonium sulfate fractionation.
r-hCK-MB was purified from crude extract by ammonium sulfate fractionation, followed by hydrophobic, anion-exchange, and gel-filtration chromatography (Table 2).