Table 2: The numbers of SSR loci and the subset of these that are potentially amplifiable
(containing suitable PCR priming sites) in 159,521unigenes using RNA-Seq
List of the amplifiable
primers for mutant detection in Rose species
Na channel is control to check quality of extracted DNA if it is amplifiable
. 16S is control to check presence of bacteria in the template DNA.
The most limiting steps while using this technique are the extractions of genomic DNA of amplifiable
quality and availability of oligonucleotides with high specificity for different species, and cross-contamination, which has been the problem in the standardization of molecular methods.
As a control to verify amplifiable
DNA or to detect the presence of PCR inhibitors in the clinical samples, a PCR designed to amplify the human gene for 0-globin was carried out as described by Bialek et al.
The Quantidex DNA assay reports amplifiable
DNA, rather than bulk DNA, and predicts performance in targeted NGS assays that rely on similar enzymatic methods to generate target-enriched, sequenceable libraries.
MiRNAs are interesting biomarkers that are functionally important, play a significant role in gene regulation, are amplifiable
, and are stable molecules.
Used with the compony's QX200 Droplet Digital PCR system, the kit provides researchers with the ability to precisely and directly measure amplifiable
A physiological exploration platform that is (1) easily constructed, (2) easily operated, (3) programmable, (4) capable of producing amplifiable
and extractable signals, and (5) noninvasive can be effectively achieved by measuring characteristics of the skin surface while applying electricity on it [1-3].
A PCR using universal primers 18ScomF1/18ScomR1 (Zhang & Lin 2005) was carried out to ensure that amplifiable
DNA was present and that a false negative would be avoided.
Furthermore, initial incubations at temperatures above and below 97[degrees]C were also tested, but no amplifiable
DNA fragment was detected in them.
The Korean group failed to detect amplifiable
mutant BRAF in any of their samples, while the Italians found mutant BRAF in all subject groups, including the normal controls.