Although the specimen exhibited enterovirus-like cytopathic effect by inoculation into HEF and GMK cells when cell culture system for virus isolation was used (2), extracted RNA from the supernatant of the cells showed no amplification
by reverse transcription--PCR (RT-PCR) when 1 of the conventional primer sets for human enteroviruses was used (3,4).
Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene products were quantified by TagMan quantitative PCR for assessment of amplification
efficiency, yield of specific amplification
products, and reproducibility of the new amplification
Bound antibodies were visualized using the three-step amplification
procedure described earlier.
Physicists have been eager for a demonstration of matter-wave amplification
because it provides a striking confirmation of quantum mechanical predictions.
Because our major objective was to enhance the quality of our residents' life by assuring presence of prescribed amplification
, systems were implemented to achieve the following specific goals:
Additional reasons for rejecting hearing aids include amplification
of background noise, limited social contacts, and difficulty with manipulation of the controls.
Nucleic acid amplification
is one of the most commonly performed molecular biology techniques and is a necessary precursor to a range of methods from gene cloning and site-directed mutagenesis to the quantitative analysis of gene expression.
COMPARATIVE ANALYSIS OF TOP ISOTHERMAL NUCLEIC ACID AMPLIFICATION
Using a real-time turbidmeter, the kit can detect the amplification
and presence of Campylobacter jejuni/coli in one hour.
Used in conjunction with expression microarrays, GenoSensor genomic microarrays can help researchers determine not only if a gene is present, but also whether the gene is found in abnormally high numbers, and if abnormal gene amplification
is correlated with overexpression of an important protein.
Intergen Company (Purchase, NY; 914-694-1700) announced the issuance of United States Patent 5,866,336 which describes the use of energy transfer labeled primers for the simultaneous amplification
and detection of nucleic acids in a closed reaction vessel.
) has announced the addition of the "Gene Amplification
Technologies - Trends & Opportunities Worldwide" report to their offering.