In those cases demonstrating MYCC or MYCN amplifications
, we reviewed the histologic characteristics to determine whether these tumors were associated with any previously described histologic patterns of anaplasia.
To validate the independent repeatability of small-scale amplifications
, we established microscale cDNA synthesis protocols (10 ng total RNA) that used independent IVT (T7) results to compare and contrast the independent 15-million-fold RFA results.
(2,3) The MLVA method involves PCR amplification
of five variable number of tandem repeats (VNTR) loci specific for S.
After 15 min, the chip was heated to 63 [degrees]C to carry out LAMP amplifications
. The prestored LAMP reagents (currently, in liquid form) included either an intercalating fluorescent dye such as EvaGreen or a colorimetric dye such as Leuco crystal violet (LCV) (30).
Objective: To review the prevalence and prognostic significance of fibroblast growth factor receptor 1 (FGFR1) amplification
and to establish an association between FGFR1 amplification
and the clinical characteristics of nonsmall cell lung cancer (NSCLC).
The most-common, potentially targetable alterations were mutations, amplifications
, and homozygous deletions of PIK3CA (n = 8; 40%), PTEN (n = 5; 25%), CDKN2A/B (n = 4; 20%), CCND3 (n = 3; 15%), CCNE1 (n = 2; 10%), and EGFR (n = 2; 10%), with AKT3, CCND1, CCND2, CDK4, FBXW7, FGFR1, HRAS, NF1, PIK3R1, and SRC altered in a single case (Figure 1; Table 1).
M2 PHARMA-April 2, 2014-Personal Genome Diagnostics unveils test for MET gene amplifications
Direct PCR amplifications
of FAW samples were each conducted in a 25 [micro]L reaction mix containing 19.4 [micro]L water (ampoule water), 5 [micro]L 5X Taq buffer (Promega), 0.2 [micro]L of dNTPs (25 [micro]M), 0.1 [micro]L of each primer JM76/JM77 (10 mM) forward JM76 -5'GAGCTGAATTAGG(G/A)ACTCCAGG3', and reverse JM77-5'ATCACCTCC(A/T)CCTGCAGGATC3' (Levy et al.
The efficiency and reliability of those technologies are often decreased by nonspecific amplifications
resulting from mishybridization of primers.
The most advanced product in X-Pol portfolio is Qualiphi, which is believed to increase the performance of DNA amplifications
Another example of nested amplifications
is provided by (11).
Genetic alterations in cervical carcinomas: Frequent low-level amplifications
of oncogenes are associated with human papillomavirus infection.