The [L.sub.V] of PGP 9.5, NPY and VIP immunostained fibres was evaluated in the Pg and Ig compartments for ventral, dorsal, ampullar and acini regions, and in the Pd compartment for the ductal region, for each age group defined above.
To obtain regional volume, the fraction of prostate volume contained in each region ([V.sub.V] amp, [V.sub.V] dor, [V.sub.V] vent and [V.sub.V] duct) was measured, i.e., the ratio between ampullar, dorsal, ventral and periurethral duct areas, and the reference area of prostate tissue.
In summary: a) discriminant analysis confirms the androgen-dependence of both neuroendocrine cells and peptidergic innervation during the postnatal development of the rat prostate; b) periglandular innervation has more relevance than interglandular innervation in order to classify rats in age groups; and the peptidergic nerves from ventral, ampullar and periductal regions are more age-dependent than the nerves from dorso-lateral regions; c) the peptidergic nerves from ventral, ampullar and periductal regions are more age-dependent than the nerves from the dorsolateral region.
For botryllids specifically, allorecognition responses at the blood level are observed as a darkening either within the cells involved or associated with blood cells that have leaked out of their ampullar
vessels and contacted cells or plasma from the opposing colony.
reptans, cellular junctions between the epithelial cells of ampullar tips became loose, and some gaps appeared (arrow in [ILLUSTRATION FOR FIGURE 8A OMITTED]).
Morula cell infiltration, which occurs in allorejection in most botryllid species, might be facilitated by the change of permeability in the ampullar epithelium (Taneda and Watanabe, 1982a).
Further evidence for this theory comes from experiments on Botrylloides fuscus, where fusion always occurs in a cut surface assay (where nonampullar vasculature elements are brought into contact), even between colonies that reject each other at the growing surface (i.e., ampullar
contacts; Hirose et al., 1994).
As previously described in detail (1), hemoblast cells are pumped into the lumen of growing ampullae by rhythmic contraction waves generated by the ampullar
walls, and hemoblast cells are often observed in the tip region of ampullae.
On the other hand, the ampullar
epithelia or some kinds of blood cells may also be alloreactive in B.
The connective tissue of the ampullar wall plays a crucial role in controlling the shape change of the ampulla (see below).
The connective tissue fibers observed in both the tube foot and the ampullar wall play a crucial role in the mechanics of the tube foot and the ampulla.