The Chromosomal changes observed (Figure 3) were mainly colchicine, disorganized metaphases, multipolar anaphases
and with loss of chromosomes.
Figure 1J-N shows the zygote at early anaphase
of the first meiosis viewed from the animal pole.
We found that Nesprin-2 was still associated with mitotic chromosomes and Nesprin-2 knockdown cells harbored increased numbers of chromatin bridges in anaphase
A: normal interphase, A1: binuclei at interphase, A2: micronucleus at interphase, B: normal prophase, B1: binuclei at prophase, B2: micronucleus at prophase, C: normal metaphase, C1: sticky metaphase, C2: C-mitosis, D: normal anaphase
, D1: anaphase
bridge, and D2: multipolar.
The wrinkling revolution process is divided into five stages: (I) prophase stage, (II) prometaphase stage, (III) metaphase stage, (IV) anaphase
stage, and (V) telophase stage.
Notably, BRG1 depletion resulted in an aberrant chromatin organization caused by a dispersion of H3K9me3 and H4K20me3 and an increased mitotic failure caused by lagging anaphase
Expression of Centromeric Sequences Induces Mis-Segregation in Anaphase
. The human (HUES-10, HT1080) and murine (E14TG2A) cells were transfected with the respective satellite expression constructs outlined above and the insert-less pIneo2 vector in a parallel control experiment.
The process of mitosis is divided into five stages: prophase, prometaphase, metaphase, anaphase
, and telophase.
TTK can directly prevent anaphase
onset in the presence of misaligned chromosomes.
Cytokinesis is achieved by an actin-myosin contractile ring that physically divides the cell into two daughter cells following separation of the sister chromatids during anaphase
. Proper positioning of the contractile ring and, hence.
In addition, the frequency of chromosomal aberrations is increased, including chromosome bridges and laggard chromosomes and leads to partition-bundle division (a novel type of chromosomal aberration, in which chromosomes at the transition from anaphase
to telophase of mitosis are divided into three, four or six bundles) (Rong et al., 2002; Gao et al., 2010).
Together with the phosphorylation of serine 10 of histone 3, which is tightly associated with the condensation of chromatin until anaphase
of mitosis, this would be indicative for G2 arrest (Hendzel et al.