A) Multipolar Anaphase
; B) Metaphase with loose chromosome; C) Metaphase colchicine; D) Anaphase
Expression of Centromeric Sequences Induces Mis-Segregation in Anaphase
. The human (HUES-10, HT1080) and murine (E14TG2A) cells were transfected with the respective satellite expression constructs outlined above and the insert-less pIneo2 vector in a parallel control experiment.
Kazic and Sofradzija (1985) studying the effects of Largaktil, a Nozinan and Valoron came to the conclusion that Nozinan significantly reduced mitotic activity and causes major changes in interphasic nuclei and the occurrence of abnormal metaphase and anaphase
. Largaktil at the start of treatment, manifest a stimulative effects, but also the appearance of nonspecific metaphases, while very strong mutagenic effects of Valoron is manifested through inhibition of mitotic activity, the occurrence of abnormal prophase, metaphase and anaphase
, and changes in the structure of chromosomes.
The chromosomes in precocious migration in metaphases I may have originated from univalents in diakinesis or have been a result of precocious terminalization of chiasma and the late terminalization of these chiasmas makes the laggard chromosomes in anaphase
I (SOUZA-KANESHIMA et al., 2010).
Normal Multipolar fragments Anaphase Anaphase
1 + - - 2 + - - 3 + - - 4 + - - 5 + - - 6 + - - 7 + - - Sample No.
It induced multipolar anaphases
and telophases and chromosome lagging.
were typical, well organized and others were amply disordered, with chromosome bridges (Figure 5a, b).
lyoni, with n = 11 or n = 12 = 11 + X, confirmed the regular reductional segregation of all the chromosomes in the preceding anaphase
I and the meta- and submetacentric morphology of the chromosomes (Figs.
Cells were observed in interphase, prophase, metaphase, anaphase
No difference in frequencies of abnormal metaphases and anaphases
was found when compared to C5-A6-T6 and C5-A8-T8 cells (Table 2).
The predominant abnormalities consisted of chromosomes/ nuclei in stickiness, bridges at anaphase
and telophase, C-metaphases, unoriented chromosomes at metaphases, and chromosomes/chromatids or even lost fragments at anaphases
(Table 4 and 5, Figure 4).
Cells in interphase, prophase, metaphase, anaphase
and telophase were observed.