The combination of silk and agar in SA sample increased the cohesion and unity of the scaffold and provided the pore system for cell attachment and cell migration of most mammalian anchorage-dependent cell
Plate colony formation assay was conducted to evaluate the effect of cantharidin on anchorage-dependent cell
Intracellular reactive oxygen species activate Src tyrosine kinase during cell adhesion and anchorage-dependent cell
Microcarrier culture introduces new possibilities allows practical high-yield culture of anchorage-dependent cell
. The advantages of using microcarrier for mammalian cells culture production, include increased productivity, reduced cost, labor-saving technique allowing the elimination of hundreds of flasks and reduced risk of contamination compared to other conventional methods.
Serum-free media (SFM) was first used in mammalian cell culture by Ham in 1965; much of the early work involved anchorage-dependent cell
Being similar to the major inorganic component of natural bone, the inorganic compound such as hydroxyapatite (HAp) or a calcium phosphate in a composite scaffold provides good osteoconductivity while the polymer provides the continuous structure and design flexibility to achieve the high porosity and high surface area, which are necessary for anchorage-dependent cells
such as osteoblasts to survive and differentiate .
Anoikis: a necessary death program for anchorage-dependent cells
Many anchorage-dependent cells
can be adapted to grow on micro-carriers to achieve high cell density.
Adhesion of cells is essential procedure to grow and functionalize anchorage-dependent cells
. Additionally, this structural similarity helps represent tissue that consists of a mass of cells by 3-dimensional culture .
Studies show that anchorage-dependent cells
growing on ECM undergo more efficient plating and have higher proliferation rates.
In the absence of the FAK signal, anchorage-dependent cells
are programmed to undergo apoptosis as a default pathway.
In the absence of a polymeric adherent surface, anchorage-dependent cells
such as HEK clump together, forming self-aggregates.