Past studies indicated the presence of anethol (Andarwulan and Shetty, 1999), anisaldehyde
(Reichling et al., 1995), coumarins (Kartnig et al., 1975), eugenol (Al Mofleh et al., 2007) and terpenes (Burkhardt et al., 1986) as abundant constituents among others which are actually responsible for scavenging of free radicals.
Heat guns are normally relegated to evaporating solvent from a TLC plate and developing reactions with visualizing reagents like phosphomolybdic acid or anisaldehyde
. In this reaction set, the simple jet of hot air allows students to quickly and controllably heat their reaction.
sulphuric acid was prepared by dissolving 5 mL of p-anisaldehyde solution in 1 ml of 98% sulphuric acid and 50 ml of acetic acid.
sulphuric acid was used as spray reagent.
After the separation, plates were observed at 254 nm and developed with anisaldehyde
(5 mL) in glacial acetic acid (50 mL) and H2SO4 (1mL).
From the previous HPLC analysis, it was showed that IVE contains anisaldehyde
and trans-anethole as main constituents .
An equimolar mixture of anisaldehyde
(10 mmol, 1.36 mL), ethyl acetoacetate (10 mmol, 1.3 mL), hydrazine hydrate (0.5 mL), and malononitrile (10 mmol, 0.66 g) was stirred for 30 min at room temperature in the presence of triethyl amine and water (5 ml).
The plates sprayed with anisaldehyde
reagent [76% methanol (Merck) and 19% orthophosphoric acid (Riedel-De Haen, Buchs, SG Switzerland), 5% p-anisaldehyde (Merck)], 30% [H.sub.2]S[O.sub.4] (Merck) solution in MeOH (Merck) and 1% vanillin-[H.sub.2]S[O.sub.4] solution [vanillin (Boehringer Mannheim, Mannheim, Germany) and [H.sub.2]S[O.sub.4] (Merck)].
10% Sodium hydroxide (15 ml) was added dropwise within 15 min to a stirring mixture of 1-vinyl-2- pyrrolidinone (1.11g) and anisaldehyde
or benzaldehyde (0.01 mole) in ethanol (30 ml).
Other substances found in anise oil are anisaldehyde
dianethole anisketone anisic acid p-methoxy acetophenone -himachalene and cis-anethole.
The sugar moieties were stained with Syldatk reagents (anisaldehyde
: sulfuric acid: glacial acetic acid, 0.5:1:50), whereas the fatty acid moieties were stained with ammonium molybdate /cerium sulfate (0.42%, w/v, ammonium molybdate and 0.2%, w/v, cerium (IV) sulfate in 6.2% sulfuric acid, Rankem, India) and the plates were heated at 120[degrees]C for 10 min.