Forty percent (eight out of 20) of the crabs examined using histology, regardless of their Hematodinium status, had a mikrocytid infection of the antennal gland, gills, and hemolymph, 15% had systemic bacterial infections and 15% had systemic fungal infections caused by Ophiocordyceps.
The cases of infection caused by a mikrocytid parasite seen in both Hematodinium-infected and noninfected crabs were characterized by the presence of free plasmodia in the lumen of the antennal gland and large numbers of uninucleate forms within the antennal gland cells (Fig.
Three of these crabs had moderate to high severity mikrocytid infections characterized by variable necrosis of the antennal gland and the presence of plasmodia and uninucleate forms of these parasites in the gills (not shown).
To determine if a similar approach can be used in Homarus americanus, activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamate dehydrogenase (GD), sorbitol dehydrogenase (SDH), amylase (AMY) and lipase (LIP) were measured in tissue homogenates of heart, hepatopancreas, abdominal muscle, proximal intestine, antennal gland, hemocyte lysate supernatant and hemolymph plasma and serum.
The activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), sorbitol dehydrogenase (SDH), glutamate dehydrogenase (GD), amylase (AMY) and lipase (LIP) was determined in homogenates of hepatopancreas, heart, abdominal muscle, proximal intestine, antennal gland, hemocyte lysate supernatant (HLS) and hemolymph plasma and serum.
Samples of hepatopancreas, heart, antennal gland, proximal intestine and abdominal muscle were collected and immediately placed on ice.
Primers to conserved SSU rRNA sequences of parasome-containing amoebae and their nonparasome-containing relatives were used to amplify overlapping SSU rRNA fragments of the presumptive parasite from gill, antenna, antennal gland and ventral nerve cord of infected lobsters.
Tissues including antenna, antennal gland, appendages, carapace, compound eye, gill, gonad, heart, hepatopancreas, intestine, mandibular apparatus and tail muscle were collected for histopathologic examination, while antenna, antennal gland, gill and ventral nerve cord were subsampled for molecular biologic analysis.
Briefly, dead and moribund lobsters from western LIS (zone 1) were collected from late October through early December 1999, and tissues including antenna, antennal gland, compound eye, hepatopancreas, gonad, stomach, intestine, gill, carapace and ventral nerve cord were fixed in 10% neutral buffered formalin, decalcified using Bouin's fixative and processed routinely for paraffin embedding.
are release structures of male antennal glands involved in courtship behaviour (Hymenoptera:Ichneumonidae).
Functional anatomy of male antennal glands in three species of Encyrtidae (Hymenoptera: Chalcidoidae).
Researchers have long known that female beewolfwasps secrete goo from their antennal glands