Conventional techniques are being replaced by modern breeding techniques like anther culture
, markers assisted selection and molecular breeding.
9] reported formation of doubled hapioid sunflower plants by androgenesis using in vitro anther culture which later turned out to be derived from diploid callus of anther wall / connective tissue rather than from anthers.
Anther culture in Helianthus annuus L, influence of genotype and culture y condiions on embryo induction and plant regeneration.
This latter method is more technically demanding, which limits its application to a range of species narrower than anther culture.
Microspore embryogenesis in anther culture of 3 species of Populus and regeneration of dihaploid plants of Populus trichocarpa.
of male-sterile tomato 9Lycopersicon esculentum Mill.
The other involves the use of anther culture
, growing plants from pollen grains.
Using a mix of conventional and advanced breeding techniques -- such as anther culture
, tissue culture, hybrid sorting, and protoplast fusion -- B&W eventually produced Y-1.
The tissue culture technique was reported by Guha and Maheshwari on Datura anther culture at first[7,1].
Produced haploid lines from microspore and anther culture provide high potential for genetic breeding with development of genetic variation through production of completely homozygous lines in short time [3,4,10,9,14,17].
Deleted forms of plastid DNA in albino plants from cereal anther culture
1990) embryogenesis from anther cultures
of Hordeum vulgaris (Cho and Kasha, 1989) and flower bud formation from thin-layer explants of Nicotiana tabacum (Smulders et al.