As basic structural unit of hypericin is emodin
anthrone, the search for possible biosynthetic routes for anthraquinone synthesis revealed the existence of an alternative pathway, through chorismate/o-succinylbenzoic acid pathway [61, 62].
Determination of protein-bound carbohydratebin serum by a modified
anthrone method.
The WSC contents were determined by colorimetric after reaction with
anthrone reagent (Thomas, 1977).
(b) Total Starch content: The total starch content was determined by
anthrone method [14].
Total carbohydrates were determined by spectrophotometric method, based on reaction between glucose and
anthrone, after hydrolysis.
Glycogen content of soft tissues was determined from April 2007 to January 2008 by the
anthrone method (Kamada & Hamada 1985).
The soluble sugar content was determined following the
anthrone method: 1 ml of a mixture of 0.2 %
anthrone in 100% [H.sub.2] S[O.sub.4] was added to 1 ml of sample and incubated in a 40[degrees]C water bath for 20 min.
At GS61 + 75[degrees]Cd (base temperature 0[degrees]C), percentage WSC content of stems and attached leaf sheaths was assessed in all plots, using the
anthrone method of Yemm and Willis (1954) as described by Gay et al.
The pellet was dried under nitrogen, then dissolved in 5 ml distilled water; the amount of glycogen in the solution was measured using the
anthrone method of Umbreit et al.
Glycogen contents were estimated in 30% potassium hydroxide solution (KOH) according to
Anthrone method of Consolazio and Lacono (1963).
Total soluble sugars were measured by
anthrone reagent (0.2%) following Sadasivam and Manickam (1992).
Nicotinamide,
anthrone, and oyster-glycogen were procured from Himedia, Mumbai, India.