avidin

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avidin

[′av·əd·ən]
(biochemistry)
A protein constituting 0.2% of the total protein in egg white; molecular weight is 70,000; combines firmly with biotin but loses this ability when subjected to heat.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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And, the successful binding of avidin-biotin conjugate was tested using the fluorescein-conjugated antibiotin antibodies under the visualization of fluorescence microscope.
An antibiotin horseradish peroxidase (HRP) will generate a tyramide radical that cross-links a biotin or a fluorophore to all exposed tyrosine residues of any protein near the recognition event (6).
Slides were then washed twice with 4x SSC-0.2% SDS buffer and 2x SSC buffer, and hybridization was detected by the sequential addition of Cy5-conjugated mouse antibiotin immunoglobulin G (IgG) (Jackson ImmunoResearch, West Grove, PA, USA) and Cy5-conjugated goat antimouse IgG (Jackson ImmunoResearch).
Following hybridization, slides were incubated successively with antibiotin antibody and fluorescein-conjugated IgG, counter-stained with propidium iodide and mounted in Vectashield medium (Vector Laboratories).
Peritoneal B-1a cells were isolated using magnetic microbeads conjugated to a cocktail of biotinconjugated antibodies against non-B-1a cells as first labeling reagent (B-1a Cell Biotin-Antibody Cocktail) and AntiBiotin MicroBeads as secondary labeling reagent (Miltenyi Biotec), following the manufacturer's instructions.
The PBMCs were magnetically labelled with a cocktail of biotin-conjugated antibodies against CD3, CD7, CD16, CD19, CD56, CD123, and Glyocophorin A and antibiotin microbeads.

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