If the amino acids in this stem antibody binding site
prove to be so important to the structure of hemagglutinin that the virus can't tolerate change there, then the virus wouldn't be able to adapt to and mutate away from a vaccine targeting this site via stimulation of neutralizing antibodies.
This mechanism of action has been validated by experiments with single-chain antibody fragments containing the antibody binding site
The new patent is much broader and now claims the use of any antibody that recognizes or binds to the epitope, an antibody binding site
on the TpP protein, that is recognized by MH-1 or 45J.
The changes turn out to be on the virus surface, suggesting very strongly, says Hogle, "that the site of the mutation represents the antibody binding site
This [K.sub.D1] value, 9.52 nmol/L, was taken as a measure for the affinity of 1 antibody binding site
. The quality of the fitting analysis was indicated by a [chi square] value of 7.8.
The latter has facilitated the development of light-scattering assays for haptens and other relatively low-molecular-mass analytes (<5000 Da and only providing one viable antibody binding site
per molecule) by using the principle of immunoinhibition .
The endogenous T3 competes with the labeled analogue T3 for capture antibody binding sites
. In contrast to sandwich assays, the signal is inversely proportional to the concentration of the analyte such as T3.
This high dose saturated the antibody binding sites
and induced falsely increased results in the competition assays for free triiodothyronine and free thyroxine and lower results in the sandwich method for thyroid-stimulating hormone.
Identification of canary grass (Phalaris aquatic) pollen allergens by immunoblotting: IgE and IgG antibody binding sites
; Allergy 1993; 48: 273-281
Practically, if one uses antibodies with known MESF values, which are assigned using the same methodology for obtaining MESF values of beads, the number of antibody binding sites
per cell is equal to the ratio of the MESF value per cell and the MESF value per antibody.
These biosensors can be applied for direct detection or in a competitive immuno-biosensor format where a labeled antigen competes with the analyte for the antibody binding sites
. The antibodies can be immobilized on fibre optic probes used in fluorescence-based systems or using surface plasma resonance, etc.