The newly allowed claims broadly cover, for example, double-stranded, RNAi-mediating molecules of between 19 and 52 nucleotides in length with a 3' overhang on the antisense strand
, and thus include siRNA trigger designs such as 'dicer substrates.' The claims also cover siRNA with one or more nucleotide analogues, including UNAs.'
HOXA-AS2 gene is located between HOXA3 and HOXA4 genes on the antisense strand
. Its transcript is expressed in human peripheral blood neutrophils and in NB4 cells, a human promyelocytic leukemia cell line derived from a patient with acute promyelocytic leukemia.
The students lined up face-to-face in two rows, one representing the DNA sense strand and the other the antisense strand
. Students were told that they were in the nucleus of the cell, the site of transcription.
The filter for the [DELTA]G (free energy) at the 5'-end of the antisense strand
The primary RNA transcript is synthesized by adding complementary base pair nucleotides in a 5[feet] to 3[feet] direction relative to the DNA antisense strand
. This event is catalyzed by DNA-dependent RNA polymerase II.
Messenger RNA works only as a single strand, but scientists can add a complementary sequence of DNA or RNA to serve as an antisense strand
. The added sequence will bind up the messenger RNA with the same powerful bonding that holds together a DNA double helix, thus creating a freak double-stranded RNA or a DNA-RNA hybrid.
The codon 6 detection probe (5'-CTCCTGTGGAGAAGTCTGC-LC Red 640) completely matched the Hb S allele antisense strand
. The codon 26 probe (LC Red 705-GTTGGTGGTAAGGCCCTGGT-phosphate) completely matched the Hb E allele antisense strand
At nt509 in CYP21P, the HaeIII restriction site (present in 30 of 40 alleles) results from the presence of G (C on the antisense strand
) at this position.
For the sense strand (FAM), the peaks were defined by the following data points: wild-type 1, 5756; wild-type 2, 5809; mutant 1, 5736; mutant 2, 5788, and for the antisense strand
(HEX): wild-type, 5867; mutant, 5738.
Interestingly, the quality of the second-round synthesis of the sense strand of the cDNA (lane 4) was equivalent to that of the first-round synthesis of the antisense strand
(lane 1) and the sense strand of the cDNA (lane 3), although the cDNA synthesis reaction did not contain random hexamer primers.
Both sense and antisense strands
of the amplified DNA was sequenced by using Big Dye terminator cycle sequencing chemistry v 3.1 (Applied Biosystems, USA) and electrophoresis was done by an automated DNA sequencer (ABI Prism 3130xL Genetic Analyzer, Applied Biosystems, USA).
Conversely, the array detected a single heterozygous mutation that was missed by conventional sequencing because the mutant signal fell below the threshold of 30% of the wild-type signal in both the sense and antisense strands
. False-positive calls were found exclusively within runs of recurrent Ns, which were not considered in calculations of assay comparability.