The newly allowed claims broadly cover, for example, double-stranded, RNAi-mediating molecules of between 19 and 52 nucleotides in length with a 3' overhang on the antisense strand
, and thus include siRNA trigger designs such as 'dicer substrates.
Since there were not enough students, the sense strand moved away, but the students were told that the opening of the helix and transcription actually occur simultaneously The sense-strand students exchanged their DNA codes for mRNA codons and moved back in line, one by one, to face the antisense strand
, demonstrating that the antisense strand
was the template for transcription.
The filter for the [DELTA]G (free energy) at the 5'-end of the antisense strand
Asymmetric amplification of the antisense strand
occurred in 10 [micro]L of 50 mmol/L Tris, pH 8.
The resulting primary RNA strand is complementary to the DNA antisense strand
and is identical to the nucleotide sequence expressed by the sense DNA strand, except that the nucleotide uridine (U) replaces T during complementary base pairing (A-U in RNA, A-T in DNA) [ILLUSTRATION FOR FIGURE 2 OMITTED].
an asymmetrical chemical modification design, where the modification in the sense strand cannot be used in the antisense strand
For the sense strand (FAM), the peaks were defined by the following data points: wild-type 1, 5756; wild-type 2, 5809; mutant 1, 5736; mutant 2, 5788, and for the antisense strand
(HEX): wild-type, 5867; mutant, 5738.
Chimeric RNA-DNA duplexes (Patent Pending): These novel RNAi-like compounds, comprised of a RNA antisense strand
and a modified DNA sense strand, were designed to provide potential specificity advantages compared with first generation siRNA.
The first product, now on the market, amplifies the antisense strand